BacT/ALERT VIRTUO和BacT/ALERT 3D两种血培养系统的性能评价  

Performance evaluation of the two blood culture systems,BacTALERT VIRTUO and BacTALERT 3D

作　　者：马艳 周春妹 黄声雷 沈佳瑾 史庆丰 潘柏申 王蓓丽 郭玮 MA Yan;ZHOU Chun-mei;HUANG Sheng-lei;SHEN Jia-jin;SHI Qing-feng;PAN Bai-shen;WANG Bei-li;GUO Wei(Zhongshan Hospital,Fudan University,Shanghai 200032,China;不详)

机构地区：[1]复旦大学附属中山医院检验科,上海200032 [2]复旦大学附属中山医院感染管理科,上海200032 [3]复旦大学附属中山医院厦门医院检验科,福建厦门361015

出　　处：《中华医院感染学杂志》2023年第11期1622-1626,共5页Chinese Journal of Nosocomiology

基　　金：上海市临床重点专科建设基金资助项目(shslczdzk03302);厦门市医疗卫生重点项目(YDZX20193502000002);病原微生物生物安全国家重点实验室开放基金资助项目(SKLPBS2144)。

摘　　要：目的对BacT/ALERT VIRTUO和BacT/ALERT 3D两种血培养系统进行性能评价,探讨病原学检出时间的差异。方法选择60株临床检出的病原菌,使用BacT/ALERT FA Plus需氧瓶(FA+)、BacT/ALERT FN Plus厌氧瓶(FN+)、BacT/ALERT FA需氧瓶(FA)和BacT/ALERT FN厌氧瓶(FN)四种血培养瓶,同时使用BacT/ALERT VIRTUO(以下简称VIRTUO)和BacT/ALERT 3D(以下简称3D)两种血培养系统进行模拟血培养实验,比较指标为不同病原菌的报阳时间。结果VIRTUO的检测时间较3D系统短0.06~16.5 h(P<0.05);FA+和FN+分别与FA和FN比较,在酵母菌中,FA+与FA的报阳时间差异有统计学意义(P=0.020);在兼性厌氧菌中,FA+与FA、FN+与FN的报阳时间差异均有统计学意义(P<0.05)。结论BacT/ALERT VIRTUO系统报阳时间短于BacT/ALERT 3D系统;FA+检测酵母菌和兼性厌氧菌快于FA,FN+检测兼性厌氧菌显著快于FN。OBJECTIVE To evaluate the performances of two blood culture system,BacT/ALERT VIRTUO and BacT/ALERT 3D,and to explore the differences in pathogenic detection times.METHODS Sixty clinically detected pathogens were selected for simulated blood culture experiment using four blood culture vials,BacT/ALERT FA Plus aerobic(FA+),BacT/ALERT FN Plus anaerobic(FN+),BacT/ALERT FA aerobic(FA),and BacT/ALERT FN anaerobic(FN),along with BacT/ALERT VIRTUO(hereinafter referred to as VIRTUO)and BacT/ALERT 3D(hereinafter referred to as 3D)blood culture system,index for comparison were time to positive reporting of different pathogenic bacteria.RESULTS The detection time for VIRTUO was 0.06-16.5 h,shorter than that of the 3D system(P<0.05).The difference in reporting time between FA+and FN+was statistically significant in yeast compared to FA and FN,respectively(P=0.020).In facultative anaerobe,the difference in reporting time between FA+and FA and FN+and FN was statistically significant(P<0.05).CONCLUSION The BacT/ALERT VIRTUO system took less time to report a positive than the BacT/ALERT 3D system.In terms of TTD,FA+detected yeast and parthenogenic anaerobes faster than FA,and FN+detected parthenogenic anaerobes significantly faster than FN.

关 键 词：血培养 病原菌 VIRTUO 3D FA Plus FN Plus 性能评价 

分 类 号：R197.324[医药卫生—卫生事业管理]