千穗谷MTP基因家族生信分析及镉胁迫下的表达特征  

Bioinformatics analysis of the MTP gene family in Amaranthus hypochondriacus L.and expression characteristics under cadmium stress

作　　者：刘霄霏 甘锦鑫 周涛 张伏中[2] 刘宁芳 卢蕊 胡龙兴[1] 徐倩 Liu Xiao-Fei;Gan Jin-Xin;Zhou Tao;Zhang Fu-Zhong;Liu Ning-Fang;Lu Rui;Hu Long-Xing;Xu Qian(Department of Pratacultural Science,College of Agronomy,Hunan Agricultural University,Changsha 410128,China;Hunan Research Academy of Environmental Sciences,Changsha 410004,China)

机构地区：[1]湖南农业大学农学院草业科学系,长沙410128 [2]湖南省环境保护科学研究院,长沙410004

出　　处：《植物科学学报》2023年第4期467-478,共12页Plant Science Journal

基　　金：湖南省研究生创新项目(CX20200674);湖南省教育厅项目(21A0126);长沙市自然科学基金项目(kq2202228)。

摘　　要：MTP(Metal tolerance protein)又称CDF(Cation diffusion facilitator),是与金属离子转运相关的一类蛋白。为了研究千穗谷(Amaranthus hypochondriacus L.)MTP基因家族的序列特征和进化关系,预测其不同成员的功能和调控机制,我们以最新的千穗谷基因组为参考,鉴定出9个MTP家族成员,分为Zn-CDF(3个)、Zn/Fe-CDF(4个)、Mn-CDF(4个)3个亚族。所有家族成员均具有MTP转运蛋白的典型结构域Cation_efflux,部分成员还包含1个ZT_dimer结构域。染色体定位显示,9个MTP基因分布在7条染色体上。Cd胁迫下,AhMTP7和AhMTP9在根和叶中的表达均被抑制,AhMTP6在叶中被抑制,在根中却被诱导;其余6个MTP基因均受Cd诱导表达,AhMTP2、AhMTP3仅在叶中被诱导,AhMTP5仅在根中被诱导。顺式作用元件分析结果显示,AhMTP启动子中存在多个逆境响应、生长发育和激素信号的顺式作用元件。miRNA预测分析结果表明,15个miRNA靶向7个AhMTP成员。Metal tolerance protein(MTP),also known as cation diffusion facilitator(CDF),is a metal ion transport-related protein.To study the sequence characteristics and evolutionary relationships of the MTP gene family in Amaranthus hypochondriacus L.,and to predict the functions and regulatory mechanisms of its members,we took the latest A.hypochondriacus genome as a reference and identified a total of nine members of the A.hypochondriacus MTP family.According to their sequence characteristics,they were divided into three subgroups:Zn-CDF(3),Zn/Fe-CDF(4),and Mn-CDF(4).All family members contained a cation_efflux domain,a typical feature of MTP transporters,with some also containing a ZT_dimer domain.Chromosome mapping showed that the nine MTP genes were distributed on seven chromosomes.Under cadmium(Cd)stress,the expression levels of AhMTP7 and AhMTP9 were inhibited in both leaves and roots,while the ex-pression level of AhMTP6 was inhibited in leaves but induced in roots.The expression levels of the other six AhMTPs were induced by Cd,with AhMTP2 and AhMTP3 only induced in leaves,and AhMTP5 only induced in roots.Promoter cis-acting element analysis identified several cis-acting elements in the AhMTP promoter for stress response,growth and development,and hormone signaling.MicroRNA(miRNA)prediction analysis showed that 15 miRNAs targeted seven AhMTPs.

关 键 词：千穗谷 MTP 基因表达 金属胁迫 基因家族 

分 类 号：Q943.2[生物学—植物学]