过表达lncRNA AC079466.1通过内质网应激信号通路对非小细胞肺癌细胞凋亡的影响  

Effects of overexpression of lncRNA AC079466.1 on apoptosis of NSCLC cells through endoplasmic reticulum stress signaling pathway

作　　者：冯梦林 卫心怡 王楠娅[2] 王萍[1] 段冷昕[1] FENG Meng-lin;WEI Xin-yi;WANG Nan-ya;WANG Ping;DUAN Leng-xin(School of Basic Medicine and Forensic Medicine,Henan University of Science and Technology,Luoyang Henan 471000,China;Cancer Center,the First Hospital of Jilin University,Changchun 130000,China)

机构地区：[1]河南科技大学基础医学与法医学院,河南洛阳471000 [2]吉林大学第一医院肿瘤中心,吉林长春130000

出　　处：《中国药理学通报》2023年第9期1689-1695,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 61701171)。

摘　　要：目的初步探讨lncRNA AC079466.1在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织和细胞中的表达,及其过表达对A549和H1299细胞增殖、凋亡、迁移、侵袭的影响。方法收集20例NSCLC患者癌组织及相应的癌旁组织,qRT-PCR检测lncRNA AC079466.1在组织和细胞中的表达。转染过表达质粒为AC079466.1组,转染空质粒为NC组,无转染为Blank组。MTT、流式细胞术、Transwell检测过表达lncRNA AC079466.1对A549和H1299细胞活力、凋亡、迁移和侵袭的影响;Western blot检测过表达lncRNA AC079466.1对内质网应激相关因子GRP78、PERK、eIF2α、ATF4、CHOP,以及Bax、caspase-3表达的影响。结果与癌旁组织相比,癌组织中lncRNA AC079466.1的表达水平明显降低;与HBE细胞相比,lncRNA AC079466.1在A549和H1299细胞的表达量明显降低。与Blank组和NC组相比,AC079466.1组A549和H1299细胞的活力、迁移、侵袭能力均明显下降,凋亡率明显升高,内质网应激相关因子GRP78、p-PERK、eIF2α、ATF4、CHOP,以及Bax、caspase-3表达均明显上调。结论过表达lncRNA AC079466.1可明显抑制A549和H1299细胞的活力、迁移和侵袭能力,并促进细胞的凋亡,其机制可能与促进内质网应激介导的细胞凋亡有关。Aim To investigate the expression of lncRNA AC079466.1 in non-small cell lung cancer(NSCLC)tissues and cells,and the effect of its overexpression on the proliferation,apoptosis,migration and invasion of A549 and H1299 cells.Methods Cancer tissues and corresponding adjacent tissues from 20 NSCLC patients were collected,and the expression of lncRNA AC079466.1 in tissue and cells was detected by qRT-PCR.AC079466.1 group was transfected with overexpression plasmid,NC group was transfected with empty plasmid,and no transfection was used in the Blank group.MTT,flow cytometry and Transwell were used to detect the effects of lncRNA AC079466.1 overexpression on the viability,apoptosis,migration and invasion of A549 and H1299 cells.Western blot was used to detect the effect of overexpression of lncRNA AC079466.1 on the expression of endoplasmic reticulum stress-related factors GRP78,PERK,eIF2α,ATF4,CHOP,Bax and caspase-3.Results Compared with adjacent tissues,the expression of lncRNA AC079466.1 in cancer tissues significantly decreased.Compared with HBE cells,the expression of lncRNA AC079466.1 significantly decreased in A549 and H1299 cells.Compared with the Blank group and NC group,the viability,migration and invasion abilities of A549 and H1299 cells in AC079466.1 group all markedly decreased,the apoptosis rate apparently increased,and the expressions of endoplasmic reticulum stress-related factors GRP78,p-PERK,eIF2α,ATF4,CHOP,Bax and caspase-3 were significantly up-regulated.Conclusion The overexpression of lncRNA AC079466.1 significantly inhibits the viability,migration and invasion of A549 and H1299 cells,and promotes cell apoptosis.The mechanism may be related to the promotion of endoplasmic reticulum stress-mediated cell apoptosis.

关 键 词：长链非编码RNA AC079466.1 非小细胞肺癌 增殖 凋亡 侵袭 内质网应激 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学] R329.28R342.2R734.2