甘蓝VQ基因家族鉴定及其响应根肿病菌侵染的表达分析  

Identification of the VQ Gene Family in Cabbage(Brassica oleracea var.capitata)and Their Expression Analysis in Response to the Infection by Plasmodiophora brassicae

作　　者：王敏[1] 陈锦秀[1] 邰翔[1] 朱晓炜 任云英[1] 薄天岳[1] WANG Min;CHEN Jin-Xiu;TAI Xiang;ZHU Xiao-Wei;REN Yun-Ying;BO Tian-Yue(Institute of Horticulture,Shanghai Academy of Agricultural Science/Shanghai Key Lab of Protected Horticultural Technology,Shanghai 201403,China)

机构地区：[1]上海市农业科学院园艺所/上海市设施园艺重点实验室,上海201403

出　　处：《农业生物技术学报》2023年第9期1786-1803,共18页Journal of Agricultural Biotechnology

基　　金：上海市科技创新行动计划农业领域项目(21N51900400);上海市农业农村委科技兴农项目(2022-02-08-00-12-F01099);国家现代农业产业技术体系建设专项资金(CARS-25);卓越团队项目(农科卓2022(005))。

摘　　要：VQ蛋白是一类包含"FxxxVQxLTG"保守基序的氨基酸序列,是植物特有的蛋白家族,参与植物生长发育和各种胁迫响应过程。为了明确甘蓝(Brassica oleracea var. capitata) VQ基因家族的生物学功能,本研究从甘蓝全基因组中共鉴定出67个甘蓝VQ基因(BoVQ),对其进行生物信息学和表达模式分析。系统发育分析表明VQ基因共划分为7个亚组。染色体定位显示BoVQ基因在甘蓝9条染色体上呈不均匀地单一或成簇分布。转录组测序数据表明BoVQ基因在甘蓝的不同组织表达存在差异。11个BoVQ基因(BoVQ10, BoVQ20, BoVQ22, BoVQ32, BoVQ36, BoVQ43, BoVQ49, BoVQ54, BoVQ59, BoVQ60和BoVQ67)参与甘蓝花发育的整个过程。实时荧光定量PCR(qRT-PCR)结果显示,BoVQ2、BoVQ12、BoVQ21、BoVQ26和BoVQ44基因在根肿病菌侵染下显著高表达。启动子顺式元件分析表明,病原菌应答元件W-box和防御与应激反应元件TC-rich repeats在响应根肿病菌(Plasmodiophora brassicae)诱导的VQ基因启动子区域富集。蛋白互作网络预测BoVQ蛋白之间存在复杂的互作关系。该研究为进一步探索BoVQ基因在甘蓝中的功能和进化提供参考。VQ protein,a class of amino acid sequences containing“FxxxVQxLTG”conservative motifs,is a plant specific protein family,which participated in plant growth,development,and various stress response.In order to clarify the biological function of the VQ gene family in cabbage,67 BoVQ genes were identified from the whole genome of cabbage,and their bioinformatics and expression patterns were analyzed.The phylogenetic analysis showed that BoVQ genes were divided into 7 subgroups.Chromosomes mapping exhibited BoVQ genes were unevenly distributed in single or cluster on 9 chromosomes of cabbage.The transcriptome data suggested that BoVQ genes expressions varied at different tissues.Eleven BoVQ genes(BoVQ10,BoVQ20,BoVQ22,BoVQ32,BoVQ36,BoVQ43,BoVQ49,BoVQ54,BoVQ59,BoVQ60 and BoVQ67)participated in the whole process of cabbage flower development.The results of real-time fluorescence quantitative PCR(qRT-PCR)showed that BoVQ2,BoVQ12,BoVQ21,BoVQ26 and BoVQ44 genes were significantly high expressed under the infection of clubroot pathogen.Prediction of cis-elements revealed that the pathogen response element W-box and the defense and stress response element TC-rich repeats were enriched in the promoter of BoVQ genes in response to Plasmodiophora brassicae.Protein interaction network prediction showed that there were complex interactions between BoVQ proteins.This study provides a reference for further exploring the function and evolution of BoVQ genes in cabbage.

关 键 词：甘蓝 VQ家族 生物信息学分析 根肿病 表达分析 

分 类 号：S635.1[农业科学—蔬菜学]