闽楠SPX基因家族的鉴定及其缺磷胁迫下的表达分析  

Identification of SPX Gene Family in Phoebe bournei and Its Expression Analysis Under Phosphorus Deficiency Stress

作　　者：吴梦洁 洪家都 李芳燕 周生财 程龙军[1] WU Meng-Jie;HONG Jia-Du;LI Fang-Yan;ZHOU Sheng-Cai;CHENG Long-Jun(College of Forestry and Biotechnology/State Key Laboratory of Subtropical Silviculture,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China;Lishui Vocational&Technical College,Lishui 323000,China)

机构地区：[1]浙江农林大学林业与生物技术学院/亚热带森林培育国家重点实验室,杭州311300 [2]丽水职业技术学院,丽水323000

出　　处：《农业生物技术学报》2023年第9期1832-1845,共14页Journal of Agricultural Biotechnology

基　　金：浙江省重点研发计划项目(2021C02054)。

摘　　要：闽楠(Phoebe bournei)是我国特有的珍贵用材树种,在南方红壤土中造林易产生低磷胁迫,影响栽培效益。挖掘闽楠耐低磷重要基因资源,对利用分子辅助育种技术培育耐低磷闽楠品种具有重要意义。SPX基因家族成员与磷吸收和平衡相关,本研究在全基因组范围内对闽楠SPX基因家族成员进行筛选、鉴定,同时,利用qPCR技术分析了PbSPX基因在闽楠根、茎和叶中的组织表达情况,以及在缺磷处理10和60 d时,PbSPX基因在植株根和叶片中的表达模式。结果显示,20个PbSPX基因分布在闽楠的1到10号染色体,根据蛋白序列特征和进化关系分属4个亚家族,包括6个SPX亚家族成员,7个SPX-EXS亚家族成员,3个SPX-MFS亚家族成员和4个SPX-RING亚家族成员。启动子顺式作用元件分析表明,PbSPX基因启动子上含有P1BS、W-box和MBS等磷胁迫响应相关元件。组织特异性分析表明,PbSPX2、PbSPXEXS1、PbSPX-RING2主要在根中表达,在叶中相对表达量较高的有12个PbSPX基因。在缺磷处理10和60 d的闽楠根中,除PbSPX6之外的其他5个SPX亚家族成员和PbSPX-MFS1都被缺磷显著诱导,与对照相比表达显著上调,而PbSPX-MFS2被显著抑制,表达显著下调。PbSPX-RING1和PbSPX-EXS1在缺磷10 d表达显著上调,PbSPX-RING3和PbSPX-EXS2在缺磷60 d表达显著上调。PbSPX-EXS6在缺磷10 d的根中表达显著下调,而在缺磷60 d时表达显著上调。叶片中PbSPX1~PbSPX5、PbSPX-EXS1及PbSPXMFS1均在缺磷60 d时被诱导,与对照相比表达显著上调,而PbSPX-MFS2在缺磷60 d表达显著下调,与根中的表达趋势一致。作为磷转运体的亚家族成员PbSPX-EXS1、PbSPX-EXS2和PbSPX-EXS6在不同组织中对磷水平信号的响应不同。结果表明,闽楠SPX亚家族成员参与了磷胁迫响应,而且其表达受相应组织中磷水平的调控。本研究初步揭示了PbSPX基因家族成员与低磷胁迫响应的关系,为进一步研究其在低磷响应中的功能和机制提供了�Phoebe bournei is a precious tree spiece unique to China.Afforestation in red loam which is very popular in south of China is prone to low phosphorus stress,affecting cultivation benefit.Exploring the gene resources in P.bournei with phosphorus tolerance is of great significance to develop P.bournei varieties with enhancing ability of low phosphorus tolerance by molecular assisted breeding.SPX gene is associated with phosphorus absorption and balance in plants.In this study,members of the SPX gene family in P.bournei were identified in the whole genome,and the expression of PbSPX gene in the roots,stems,and leaves of P.bournei were analyzed using qPCR,as well as the expression patterns in the roots and leaves of P.bournei after 10 and 60 d of phosphorus deficiency treatment.The results showed that 20 PbSPXs were distributed on chromosomes 1 to 10 of P.bournei.According to characteristics of protein and phylogenetic tree,PbSPXs were divided into 4 subfamilies,including 6 members of SPX subfamily,7 members of SPX-EXS subfamily,3 members of SPX-MFS subfamily and 4 members of SPX-RING subfamily.Analysis of promoter cis-acting elements showed that PbSPX genes containing elements related to phosphorus stress response,such as P1BS,W-box and MBS.Tissue specific expression analysis showed that PbSPX2,PbSPX-EXS1 and PbSPX-RING2 were mainly expressed in roots,and another 12 PbSPXs were expressed highly in leaves.In roots,when treated with phosphorus deficiency after 10 and 60 d,the expression of PbSPX-MFS1 and all PbSPX subfamily members except for PbSPX6 were induced and the expression of them were significantly upregulated compared to the control,however,the expression of PbSPX-MFS2 was significantly down-regulated.At the same time,the transcript levels of PbSPX-RING1 and PbSPX-EXS1 were significantly increased at 10 d of phosphorus deficiency treatment,while the expressions of PbSPX-RING3 and PbSPX-EXS2 were significantly up-regulated until after 60 d of phosphorus deficiency treatment.The expression of PbSPX-EXS6 was si

关 键 词：闽楠 SPX基因家族 基因表达 缺磷 

分 类 号：S718.46[农业科学—林学]