NGF及其受体在牦牛与黄牛间脑及脑干不同区域中的表达与分布  

Expression and Distribution of NGF and Its Receptor in Different Regions of Diencephalon and Brainstem of Yaks(Bos grunniens)and Cattles(B.taurus)

作　　者：吴亚娟 杜晓华[1] 刘霞[2] 郑丽平 刘珊珊[1] WU Ya-Juan;DU Xiao-Hua;LIU Xia;ZHENG Li-Ping;LIU Shan-Shan(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]甘肃农业大学生命科学技术学院,兰州730070

出　　处：《农业生物技术学报》2023年第9期1857-1867,共11页Journal of Agricultural Biotechnology

基　　金：甘肃农业大学科技创新基金-青年导师扶持基金(GAU-QDFC-2021-03);国家自然科学基金(31760305)。

摘　　要：神经生长因子(nerve growth factor, NGF)主要通过结合其高亲和力受体酪氨酸蛋白激酶A(tyrosine protein kinase A, TrkA)在中枢神经系统组织或细胞低氧缺血适应性调控方面发挥重要的生理作用。本研究旨在阐明牦牛(Bos grunniens)适应高寒低氧环境过程中NGF和TrkA在间脑及脑干不同区域的表达及定位,并探讨二者之间的相互作用关系。分别采取不同海拔地区牦牛与黄牛(B. taurus)间脑及脑干组织,采用qPCR、Western blot、免疫组织化学技术(immunohistochemistry, IHC)对NGF及TrkA的表达水平及分布特征进行比较研究。结果显示,NGF和TrkA基因及蛋白在牦牛与黄牛间脑及脑干不同区域组织中均有表达,且表达存有差异。NGF基因及蛋白在牦牛与黄牛丘脑中表达量最高,显著高于其他组织(P<0.05);牦牛丘脑、延髓中NGF基因及蛋白水平显著高于黄牛(P<0.05),但在下丘脑和脑桥中均低于黄牛;而TrkA基因水平在丘脑、脑桥、延髓中均显著高于黄牛(P<0.05),在下丘脑中与黄牛无显著差异;TrkA蛋白水平在丘脑、下丘脑、脑桥及延髓中均与黄牛无显著差异。NGF和TrkA蛋白在牦牛与黄牛间脑及脑干中的分布与定位特征基本一致,在丘脑、下丘脑中NGF和TrkA蛋白主要定位于多形细胞胞质中,脑桥中主要定位于运动神经元,延髓中主要定位于神经元胞质。以上结果提示,下丘脑及脑桥对低氧比较耐受,丘脑及延髓对低氧比较敏感,通过激活相关的神经保护机制上调NGF表达增强牦牛丘脑与延髓对低氧的耐受性,而TrkA在低氧条件下发挥作用不显著。NGF及TrkA主要定位于间脑及脑干各区域中的神经元胞质及神经胶质细胞,说明二者协同对神经元及神经胶质细胞发挥内源性神经保护作用,进而保护脑组织。本研究为进一步探究牦牛脑组织低氧适应机制提供了理论依据与数据参考。By connecting to its high affinity receptor tyrosine protein kinase A(TrkA),nerve growth factor(NGF)plays a crucial physiological role in the adaptive regulation of hypoxic ischemia in central nervous system tissues or cells.The purpose of this study was to investigate the expression,distribution and interaction of NGF and TrkA during adaptation to low oxygen environments in the diencephalon and brainstem of yaks(Bos grunniens).Different areas of the diencephalon and brainstem of yaks and cattles(B.taurus)were collected at different altitudes,and the expression levels and distribution features of NGF and TrkA were examined using qPCR,Western blot,and immunohistochemistry(IHC)methods.The results showed that the expression levels of NGF and TrkA genes and protein were significantly higher in the thalamus of yaks and cattles diencephalon and brainstem than in other tissues(P<0.05);Compared with cattles,NGF gene and protein expression levels in the thalamus and medulla oblongata of yaks were significantly higher than in cattles(P<0.05),but lower in the hypothalamus and pons.There was no significant difference between cattle and yaks in the hypothalamus,but there were considerably increased TrkA gene expression levels in the thalamus,pons,and medulla oblongata in yaks(P<0.05).The levels of TrkA protein in the cantamus,hypothalamus,pons,and medulla oblongata were not significantly different from those of cattle.The distribution characteristics of TrkA in the yaks diencephalon and brainstem coincided with NGF,and the intensity of the immunopositive response to TrkA protein was higher than that of NGF protein.The positive products of NGF and TrkA were primarily distributed in the cytoplasm of pleomorphic cells in the thalamus and hypothalamus,motor neurons in the pons,and neurons in the medulla oblongata.According to the aforementioned findings,the thalamus in the diencephalon and brainstem of yaks was the region of the brain that was most vulnerable to hypoxia.The above results showed that the hypothalamus and pons were

关 键 词：神经生长因子(NGF) 酪氨酸蛋白激酶A(TrkA) 牦牛 间脑 脑干 

分 类 号：S852.1[农业科学—基础兽医学]