FSH来源及其剂量对西藏阿旺绵羊超数排卵效果的比较  

Comparative on Superovulation Effects with FSH Source and Its Dosage on Tibet Awang Sheep(Ovis aries)

作　　者：李涛 王幸栓 虞莲 杨志勇 林耀民 高志敏[1] 李建强 雷安民[1] LI Tao;WANG Xing-Shuan;YU Lian;YANG Zhi-Yong;LIN Yao-Min;GAO Zhi-Min;LI Jian-Qiang;LEI An-Min(College of Veterinary Medicine/Shaanxi Stem Cell Engineering and Technology Research Center,Northwest A&F University,Yangling 712100,China;Changdu Jinken Farming Technology Co.,Ltd.,Changdu 854000,China)

机构地区：[1]西北农林科技大学动物医学院/陕西省干细胞工程技术研究中心,杨凌712100 [2]西藏昌都津垦牧业科技有限公司,昌都854000

出　　处：《农业生物技术学报》2023年第9期1890-1898,共9页Journal of Agricultural Biotechnology

基　　金：陕西省重点研发计划项目(2020NY-011);昌都市政府项目:阿旺绵羊种质资源保护项目(横向)。

摘　　要：阿旺绵羊(Ovis aries)作为西藏高原地区特有的珍稀羊品种资源,具有独特的风味、优质的产肉性能以及较强的高原适应能力。为了防止阿旺绵羊存栏量下降和保护当地种质资源,本研究通过同期发情、超数排卵、手术法回收胚胎等方法,分析了国产和进口促卵泡激素(follicle-stimulating hormone, FSH)来源及其剂量、超数排卵次数和有无发情史对阿旺绵羊超排效果的影响。结果表明,国产FSH对阿旺绵羊的超数排卵效果最佳,黄体平均数和有效胚胎平均数显著高于进口FSH组和国产+进口FSH组(P<0.05),可用胚平均数和胚胎回收率无差异。270和500 IU的FSH对阿旺绵羊超数排卵效果相同,分别对>50和40~50 kg的阿旺绵羊的超数排卵效果影响不显著;有无发情史对超数排卵效果无显著影响。另外,首次经过超数排卵的阿旺绵羊的总黄体数和回收胚数极显著高于多次超数排卵组(P<0.01)。因此,270 IU的国产FSH对阿旺绵羊超数排卵效果最好。本研究可以为当地阿旺绵羊保种和扩繁工作提供参考。Awang sheep(Ovis aries),as a special and precious sheep breed resource in the Tibet Plateau,has unique flavors,high-quality meat production and strong plateau adaptability.To prevent the decline of Awang sheep in stock and to protect local germplasm resources,this study analyzed the effects of different folliclestimulating hormone(FSH)sources and its doses of FSH,superovulation times and the estrus history on the effect of superovulation in Awang sheep by synchronous estrus,superovulation and surgical embryo recovery.The results showed that domestic FSH had the best effect on superovulation in Awang sheep.The average number of corpus luteum and average effective embryos in domestic FSH group were significantly higher than those in the imported FSH group and the domestic+imported FSH group(P<0.05).There was no significant difference in the average number of available embryos and embryo recovery rate(P>0.05).The 270 and 500 IU of domestic FSH had the same effect on superovulation in Awang sheep,and had no significant effect on the superovulation effect of Awang sheep weighing(>50 kg and 40~50 kg),respectively.There was no significant effect of oestrus history on the effect of superovulation.In addition,the total number of corpus luteum and the number of recovered embryos in the first superovulation group were extremely significant higher than those in the multiple superovulation(P<0.01).In conclusion,270 IU of domestic FSH had the best effect on superovulation in Awang sheep,which can be used as a reference for the conservation and propagation of local Awang sheep.

关 键 词：阿旺绵羊 超数排卵 促卵泡激素(FSH) 

分 类 号：S826[农业科学—畜牧学]