长江刀鲚FABP7基因遗传多态性及其与生长性状的相关分析  

Genetic Polymorphisms of FABP7 Gene and Their Correlative Analysis with Growth Traits in Coilia ectenes

作　　者：于爱清 施永海 严银龙 YU Ai-Qing;SHI Yong-Hai;YAN Yin-Long(Shanghai Fisheries Technology Extension Station,Shanghai Fisheries Research Institute,Shanghai 200433,China)

机构地区：[1]上海市水产研究所上海市水产技术推广站,上海200433

出　　处：《农业生物技术学报》2023年第9期1899-1913,共15页Journal of Agricultural Biotechnology

基　　金：上海市自然科学基金(19ZR1477900);上海市科技兴农重点攻关项目(沪农科攻字(2016)第6-2-2号)。

摘　　要：长江刀鲚(Coilia ectenes)是我国重要的经济鱼类,目前其自然种质资源急剧衰退,探究其脂肪酸结合蛋白7(C. ectenes fatty acid-binding proteins 7, CeFABP7)基因连锁SNP位点与其生长性状的相关性,对于其生长性状的遗传改良及新品种的培育具有重要作用。本研究基于长江刀鲚的转录组数据,利用基因克隆技术获取CeFABP7基因的cDNA全长序列和基因组DNA序列,对其核心选育F3代群体的20个具有极端生长表型个体的CeFABP7基因全长序列直接测序,筛选获得该基因上的SNP位点后,再利用直接测序法或SnaPshot法对同批次随机选取的100尾长江刀鲚进行基因分型,最后通过最小二乘法分析基因多态性与长江刀鲚生长性状的相关性。结果显示,该基因cDNA全长1 295 bp,ORF由399个核苷酸组成,编码132个氨基酸;基因组DNA全长1 899 bp,包括4个外显子和3个内含子;共检测到5个SNP突变位点和1个TGT插入/缺失突变(I/D),分布于5’-非编码区、第1内含子和第3内含子;位点g.276C>G与长江刀鲚的体长、体高和体质量性状均显著相关(P<0.05),位点g.306A>C、g.747C>T和g.825C>T与长江刀鲚的体质量和体高性状显著相关(P<0.05),位点g.1447A>T和g.1532I>D由于部分基因型个体数量较少,显示与生长性状的相关性不显著;6个位点对长江刀鲚养殖群体遗传分析显示,长江刀鲚养殖群体的平均观测杂合度(observed heterozygosity, Ho)、平均期望杂合度(expected heterozygosity, He)和平均多态信息含量(polymorphic information of content, PIC)分别为0.257、0.339和0.275。长江刀鲚FABP7基因上的位点g.276C>G有望成为剔除劣质长江刀鲚繁育亲本的重要候选分子标记,本研究对长江刀鲚的分子标记辅助育种具有理论指导意义。As an important economic fish in China,the natural germplasm resources of Coilia ectenes are declining rapidly.Exploring the correlation between genetic polymorphisms of C.ectenes fatty acid-binding proteins 7(CeFABP7)gene with the growth traits plays an important role in the breeding and improvement of growth traits of C.ectenes.Based on the transcriptome data of C.ectenes and gene cloning technology,the cDNA and DNA sequences of CeFABP7 were obtained.The SNPs of CeFABP7 gene was screened by DNA direct sequencing method using 20 individuals that selected from the F3 generation population of C.ectenes with extreme growth characteristics.Then 100 individuals that randomly selected from the same population of C.ectenes were genotyped by DNA direct sequencing or SnaPshot method.The relationship between these SNPs and growth traits of C.ectenes was analyzed by least squares method.The cDNA length of CeFABP7 was 1295 bp,containing an open reading frame of 399 bp.The genomic DNA of CeFABP7 was 1899 bp in length containing 3 introns and 4 exons.Six polymorphic sites were detected in 5′-UTR(2),intron 1(2)and intron 3(2).The locus g.276C>G was significantly associated with the body length,body height and body weigh traits of C.ectenes(P<0.05),the locus g.306A>C,g.747C>T and g.825C>T were significantly associated with body height and body weigh traits of C.ectenes(P<0.05),while the locus g.1447A>T and g.1532I>D were not significantly associated with 3 growth traits of C.ectenes(P>0.05).The result of population genetic analysis of cultured population of C.ectenes basing on 6 mutation locus showed that the average value of observed heterozygosity(Ho),expected heterozygosity(He)and polymorphism information content(PIC)were 0.257、0.339 and 0.275,respectively.The locus g.276C>G in the CeFABP7 gene was expected to be an important candidate molecular marker for eliminating inferior breeding parents of C.ectenes.This study has theoretical instructive significance for molecular marker assisted breeding in C.ectenes.

关 键 词：长江刀鲚 脂肪酸结合蛋白7(FABP7)基因 生长性状 单核苷酸多态性 关联分析 

分 类 号：S937.3[农业科学—渔业资源]