广东番木瓜曲叶病毒和一品红曲叶病毒致病性分析  

Pathogenicity Analysis of Papaya leaf curl Guangdong virus and Euphorbia leaf curl virus

作　　者：李景远 林文忠 李丁山 马崇欢 吴祖建[1] 杜振国[1] 张洁[1,2] LI Jing-Yuan;LIN Wen-Zhong;LI Ding-Shan;MA Chong-Huan;WU Zu-Jian;DU Zhen-Guo;ZHANG Jie(Institute of Plant Virology/State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Beijing 100193,China)

机构地区：[1]福建农林大学植物病毒研究所/闽台作物有害生物生态防控国家重点实验室,福州350002 [2]植物病虫害生物学国家重点实验室,北京100193

出　　处：《农业生物技术学报》2023年第9期1935-1943,共9页Journal of Agricultural Biotechnology

基　　金：植物病虫害生物学国家重点实验室开放基金(SKLOF202108);福建农林大学科技创新专项(CXZX2020017A)。

摘　　要：近年来,广东番木瓜曲叶病毒(Papaya leaf curl Guangdong virus, PaLCuGDV)和一品红曲叶病毒(Euphorbia leaf curl virus, EuLCV)经常在百香果(Passiflora edulis)上被鉴定。本研究从福建地区表现典型双生病毒危害症状的百香果上扩增得到该2种病毒的全长序列,分别为2 732和2 745 bp;为明确PaLCuGDV和EuLCV的致病性,利用同源重组技术构建了2种病毒的侵染性克隆,通过农杆菌(Agrobacterium tumefaciens)介导法接种本氏烟(Nicotiana benthamiana),结果发现,接种EuLCV的本氏烟表现出明显矮化且顶端叶片畸形卷曲症状,受病害危害明显重于接种PaLCuGDV,后者仅表现新叶稍微卷曲和叶片黄化;2种病毒混合侵染,受病害危害重于单独接种EuLCV或PaLCuGDV。Southern blot结果显示,PaLCuGDV和EuLCV单独及混合接种的烟草均有较高的病毒积累量。本研究首次构建了PaLCuGDV的侵染性克隆,并发现EuLCV和PaLCuGDV复合侵染会加重病害症状。本研究为解析PaLCuGDV和EuLCV的致病机制提供参考。In recent years,Papaya leaf curl Guangdong virus(PaLCuGDV)and Euphorbia leaf curl virus(EuLCV)were usually identified in Passiflora edulis.The full-length sequences of the 2 viruses,2732 and 2745 bp respectively,were amplified from Passiflora edulis showing typical geminiviral damage symptoms in Fujian province.In order to determine the pathogenicity of PaLCuGDV and EuLCV,the infectious clones of 2 viruses were constructed by homologous recombination technology and Nicotiana benthamiana plants were inoculated with Agrobacterium-mediated method.The results showed that the symptoms of N.benthamiana inoculated with EuLCV were obviously dwarfed and the top leaves were deformed and curled,which was significantly heavier than that of PaLCuGDV,while the latter only showed slightly curly new leaves and yellowing leaves.Further more,the symptoms of mixed infection of the 2 viruses was much heavier than any of the symptoms induced by PaLCuGDV or EuLCV alone.Southern blot results showed that N.benthamiana inoculated with PaLCuGDV and EuLCV alone or in combination had high virus accumulation.In this study,the infectious clone of PaLCuGDV was constructed for the first time,and it was found that the mixed infection of EuLCV and PaLCuGDV could aggravate the induced symptoms.This study provides a reference for understanding the pathogenic mechanism of PaLCuGDV and EuLCV.

关 键 词：广东番木瓜曲叶病毒(PaLCuGDV) 一品红曲叶病毒(EuLCV) 侵染性克隆 百香果 

分 类 号：S432.1[农业科学—植物病理学]