聚维酮碘对家兔创面组织浸泡冲洗的影响  

Effect of povidone-iodine on the immersion and flushing of wound tissue in rabbits

作　　者：张强[1] 许岩[1] 马壮壮 张昊[2] 李子豪 刘森涵 陈伟[2] Zhang Qiang;Xu Yan;Ma Zhuangzhuang;Zhang Hao;Li Zihao;Liu Senhan;Chen Wei(The First Hospital of Jilin University,Changchun 130000,Jilin Province,China;Department of Orthopedics,China-Japan Union Hospital of Jilin University,Changchun 130033,Jilin Province,China)

机构地区：[1]吉林大学第一医院,吉林省长春市130000 [2]吉林大学中日联谊医院骨科,吉林省长春市130033

出　　处：《中国组织工程研究》2024年第23期3669-3673,共5页Chinese Journal of Tissue Engineering Research

基　　金：吉林省卫生科研人才专项项目(2021SCZ03),项目负责人:陈伟。

摘　　要：背景:临床上人工关节置换所面临的最危险、最严重的并发症就是发生人工关节假体周围感染,找到一个能够预防人工关节置换假体周围感染的方法是迫切需要的。目的:观察聚维酮碘对家兔肌肉、血管、脂肪和骨质等组织进行浸泡冲洗后的影响。方法:选取40只10周龄雄性新西兰大白兔,每只兔子的左后腿作为实验组,右后腿作为对照组。兔后肢麻醉后切开,显露后肢的肌肉、血管、脂肪和骨质,对照组手术切口内用生理盐水浸泡和冲洗;实验组手术切口内用聚维酮碘原液及生理盐水浸泡和冲洗。40只兔分别在浸泡聚维酮碘0,1,3,5 min时随机处死10只切取创面组织标本,采用苏木精-伊红染色制片,对细胞数目及分布进行统计学分析和比较。结果与结论:①经聚维酮碘浸泡冲洗的肌肉、血管、脂肪和骨质与生理盐水对照组相比,镜下未见其细胞结构、形态及数目有明显的差异;②运用配对t检验探究对照组与实验组之间的差异性,配对数据均没有呈现出差异性(P>0.05);③提示聚维酮碘对家兔肌肉、血管、脂肪和骨质等组织进行浸泡冲洗后与生理盐水相比未见明显差异,反映了聚维酮碘溶液作为创口内消毒剂的安全有效性。BACKGROUND:Clinically,the most dangerous and serious complication of artificial joint replacement is periprosthetic infections.It is urgent to find a way to prevent periprosthetic infections after artificial joint replacement.OBJECTIVE:To study the effect of povidone-iodine on muscle,blood vessel,fat and bone of rabbits after immersion and flushing.METHODS:Forty male New Zealand rabbits aged 10 weeks were selected.The left hind leg of each rabbit served as the experimental group and the right hind leg served as the control group.After anesthesia,the hind limbs of each rabbit were cut open to expose the muscle,blood vessels,fat and bone.The control group was soaked and flushed with normal saline inside the surgical incision,while the experimental group was soaked and flushed with povidone-iodine inside the surgical incision.After being soaked in povidone-iodine for 0,1,3,5 minutes,10 rabbits were randomly selected and executed to collect wound tissue samples.The samples were made into pathological slices for hematoxylin-eosin staining observation as well as statistical analysis and comparison of cell counts.RESULTS AND CONCLUSION:Compared with the control group,the muscle,blood vessels,fat and bone after immersion and flushing with povidone-iodine showed no obvious difference in cell structure,morphology and number under microscope.The paired t-test was used to explore the difference between the control and experimental groups,and the paired data did not show any difference(P>0.05).It is suggested that povidone-iodine shows no significant difference from normal saline after immersion and flushing of rabbit tissues such as muscle,blood vessels,fat and bone,indicating that povidone-iodine solution as an intra-incisional antiseptic is safe and effective.

关 键 词：聚维酮碘 创面组织 浸泡 冲洗 关节置换 假体周围感染 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R394.2