漏芦通过TLR4/NF-κB通路抑制脂多糖诱导的小鼠乳腺上皮细胞炎症反应  

Inbition of Lipopolysaccharids-Induced Inflammation in Mouse Mammary Epithelial Cells by Rhaponticum uniflorum(L.)DC.via TLR4/NF-κB Pathway

作　　者：刘莉莉[1] 陈敏 LIU Lili;CHEN Min(College of Pharmacy,Heilongjiang University of Chinese Medicine,Heilongjiang Harbin 150040,China)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《饲料工业》2023年第17期92-97,共6页Feed Industry

基　　金：国家自然科学基金项目[82003930];黑龙江中医药大学基金项目[15041200003]。

摘　　要：为探讨漏芦醇提物(RUEE)对脂多糖(LPS)诱导的小鼠乳腺上皮细胞(HC11细胞)的抗炎作用及机制,试验利用RUEE(80μg/mL)、LPS(1μg/mL)单独处理以及RUEE(80μg/mL)+LPS(1μg/mL)共处理HC11细胞,采用荧光定量PCR检测炎性细胞因子及Toll样受体4(TLR4)mRNA表达水平,采用Western blotting检测核转录因子κB(NF-κB)通路关键因子的蛋白表达量。结果表明:LPS诱导可明显提高HC11细胞肿瘤坏死因子-α(TNF-α)、环氧合酶-2(COX-2)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)的mRNA表达水平(P<0.05);明显上调TLR4 mRNA表达及NF-κB p65和NF-κB抑制蛋白α(IκBα)的磷酸化水平(P<0.05)。RUEE预处理可显著降低LPS诱导的HC11细胞TNF-α、COX-2、IL-6、IL-1β的mRNA表达(P<0.05);显著下调TLR4 mRNA表达及NF-κB p65和IκBα的磷酸化水平(P<0.05)。由此可知漏芦醇提物可通过抑制TLR4/NF-κB通路的激活,下调相关炎性细胞因子的过度表达而缓解LPS诱导的乳腺上皮细胞炎症反应。In order to explore the anti-inflammatory effect and mechanism of RUEE on LPS-induced mouse mammary epithelial cells(HC11 cells),HC11 cells were treated with RUEE(80μg/mL),LPS(1μg/mL)and RUEE(80μg/mL)+LPS(1μg/mL)co-treatment.The mRNA expression level of inflammatory cytokines and TLR4 were detected by fluorescence quantitative PCR,and the protein expression of key factors of NF-κB pathway were detected by Western blotting.The results showed that LPS induction could significantly increase the mRNA expression level of TNF-α,COX-2,IL-6,and IL-1βin HC11 cells(P<0.05),obviously up-regulate TLR4 mRNA expression and the phosphorylation level of NF-κB p65 and IκBα(P<0.05).RUEE pretreatment could dramatically reduce the mRNA expression of TNF-α,COX-2,IL-6,and IL-1βin HC11 cells induced by LPS(P<0.05),evidently down-regulate TLR4 mRNA expression and the phosphorylation level of NF-κB p65 and IκBα(P<0.05).It shows that RUEE can inhibit the activation of TLR4/NF-κB pathway to downregulate the overexpression of related inflammatory cytokines and alleviate the LPS-induced inflammatory response of mammary epithelial cells.

关 键 词：漏芦 乳腺上皮细胞 脂多糖 炎症反应 NF-ΚB信号通路 

分 类 号：S813.3[农业科学—畜牧学]