腐烂茎线虫分子鉴定及在不同寄主上繁殖力分析  

Molecular identification of Ditylenchus destructor and fertility analysis on different hosts

作　　者：毛彦芝[1] 朱梓绮 王文重[1] 李庆全[1] 董学志[1] 魏琪[1] 郭梅[1] 闵凡祥[1] 杨帅[1] MAO Yanzhi;ZHU Ziqi;WANG Wenzhong;LI Qingquan;DONG Xuezhi;WEI Qi;GUO Mei;MIN Fanxiang;YANG Shuai(Institute of Industrial Crops of Heilongjiang Academy of Agricultural Sciences/Heilongjiang Provincial Key Laboratory of Potato Biology and Quality Improvement,Harbin 150086,China;Heilongjiang University,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院经济作物研究所/黑龙江省马铃薯生物学与品质改良重点实验室,黑龙江哈尔滨150086 [2]黑龙江大学,黑龙江哈尔滨150086

出　　处：《土壤与作物》2023年第3期336-342,共7页Soils and Crops

基　　金：黑龙江省博士后科研启动金项目(LBH-Q21175);黑龙江省农业科学院基金培育项目(2019JJPY015);国家马铃薯产业技术体系(CARS-09-P04).

摘　　要：腐烂茎线虫危害马铃薯在我国多地已有发生,为了明确腐烂茎线虫的生物型及在不同寄主上的繁殖能力,本研究采用贝曼漏斗法对线虫进行分离,根据国标GB/T 29577-2013描述方法对分离到线虫群体进行分子鉴定。从NCBI选取马铃薯和甘薯的腐烂茎线虫ITS序列,进行系统发育分析,利用胡萝卜愈伤组织进行培养,用玉米、马铃薯、高粱、大豆和小麦测定腐烂茎线虫在不同寄主上的繁殖能力。结果显示:分离到的线虫为腐烂茎线虫B型,来自甘薯和马铃薯寄主腐烂茎线虫明显分为2支,推断腐烂茎线虫的进化受寄主的影响。该线虫群体在参试寄主上都能生长,在小麦上繁殖系数最低,其次为大豆。种植小麦和大豆可能对腐烂茎线虫有抑制作用。The damage of Ditylenchus destructor to potato has occurred in many places in China.In order to identify the biotype of D.destructor and its reproductive capacity on different hosts,the nematodes were isolated by Baermann funnel method and identified by molecular method according to the description of GB/T 29577-2013.The ITS sequences of D.destructor from potato and sweet potato were selected from NCBI for phylogenetic analysis.D.destructor was cultured with carrot callus and then inoculated into the roots of corn,potato,sorghum,soybean and wheat to determine its reproductive capacity.The results showed that the isolated D.destructor was type B.The genetic evolution analysis showed that D.destructor from sweet potato and potato hosts was obviously divided into two branches.It would be inferred that the evolution of D.destructor was affected by the host.D.destructor reproduced on all the tested hosts.The reproduction coefficient of D.destructor on wheat was the lowest,followed by soybean.Planting wheat and soybean may have inhibitory effect on D.destructor.

关 键 词：腐烂茎线虫 扩繁 寄主 

分 类 号：S432.6[农业科学—植物病理学]