广东省中山市非O1/非O139群霍乱弧菌分子特征及耐药性  

Molecular characteristics and drug resistance of non-O1/non-O139 Vibrio cholerae in Zhongshan City,Guangdong Province

作　　者：仇绮琳 郑悦康[1] 区金结 刘绮明 吴灿权[1] QIU Qi-lin;ZHENG Yue-kang;OU Jin-jie;LIU Qi-ming;WU Can-quan(Zhongshan Center for Disease Control and Prevention,Zhongshan,Guangdong 528403,China)

机构地区：[1]中山市疾病预防控制中心,广东中山528403

出　　处：《中国热带医学》2023年第6期619-624,共6页China Tropical Medicine

基　　金：中山市医学科研项目(No.2022J206)。

摘　　要：目的了解中山市非O1/非O139群霍乱弧菌的分子特征以及耐药情况,为霍乱防治提供实验室依据。方法收集中山市2015—2021年分离自散发患者及水产品监测的非O1/非O139群霍乱弧菌,采用基质辅助激光解析电离飞行时间质谱法(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF-MS)对菌株进行鉴定及图谱聚类分析,采用实时荧光定量聚合酶链反应(polymerase chain reaction,PCR)法对菌株进行ctxA毒力基因检测,采用脉冲场凝胶电泳法(pulsed-field gel electrophoresis,PFGE)对菌株进行聚类分析,采用微量肉汤稀释法对菌株进行药物敏感试验。结果2015—2021年中山市共分离出33株非O1/非O139群霍乱弧菌,其中28株分离自散发患者,5株分离自水产品。经MALDI-TOF-MS鉴定,33株非O1/非O139群霍乱弧菌均能鉴定到种的水平,鉴定结果均为霍乱弧菌。33株非O1/非O139群霍乱弧菌中,有1株携带ctxA毒力基因,耐药菌株占69.7%(23/33),多重耐药菌株占18.2%(6/33),共产生7种耐药谱,其中3种为多重耐药谱,对8种试验药物存在耐药现象,其中对链霉素、头孢唑林、复方新诺明的耐药率均超过30%。33株非O1/非O139群霍乱弧菌分为32个PFGE型别,各型别相似度为61.7%~100%,MALDI-TOF-MS图谱聚类分析将33株非O1/非O139群霍乱弧菌分为两大聚类。结论中山市非O1/非O139群霍乱弧菌分子分型结果呈多样性,PFGE和MALDI-TOF-MS图谱聚类分析之间未发现明显相关性。菌株对部分抗菌药物存在不同程度的耐药现象,存在多重耐药菌株和产毒株,应警惕非O1/非O139群霍乱弧菌的危害性并加强监测。Objective To investigate the molecular characteristics and drug resistance of non-O1/non-O139 Vibrio cholerae in Zhongshan City,and to provide laboratory basis for cholera prevention and control.Methods The strains of nonO1/non-O139 Vibrio cholerae isolated from sporadic patients and aquatic products from 2015 to 2021 in Zhongshan city were collected.The identification and cluster analysis of the strains were analyzed by matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS),the ctxA virulence gene of strains were detected by real-time fluorescence quantitative PCR,the cluster analysis of the strains was analyzed by pulsed-field gel electrophoresis(PFGE),and the drug resistance of the strains were analyzed by microbroth dilution method.Results From 2015 to 2021,33 strains of non-O1/non-O139 Vibrio cholerae were isolated from Zhongshan City,including 28 strains from sporadic patients and 5 strains from aquatic products.Through MALDI-TOF-MS identification,33 strains of non-O1/non-O139 Vibrio cholera can be identified to the level of species,and the identification results were all Vibrio cholerae.Among 33 non-O1/non-O139 Vibrio cholerae strains,1 strain carried the ctxA virulence gene.The drug-resistant strains accounted for 69.7%(23/33),and the multidrug resistant strains accounted for 18.2%(6/33).A total of 7 kinds of drug resistance spectrum were produced,including 3 kinds of multidrug resistant spectrum,and showed drug resistance to 8 antibiotics,among which the resistance rates to streptomycin,cefazolin and compound sulfamethoxazole were above 30%.The 33 strains of non-O1/non-O139 Vibrio cholerae were divided into 32 PFGE fingerprints with a similarity ranging from 61.7%to 100%.MALDI-TOF-MS cluster analysis divided 33 nonO1/non-O139 Vibrio cholerae strains into two clusters.Conclusions The results of molecular typing of non-O1/non-O139 Vibrio cholerae in Zhongshan City presented diversity,and no significant correlation was found between PFGE and MALDITOF-MS cluster analysis.T

关 键 词：非O1/非O139群霍乱弧菌 ctxA毒力基因 脉冲场凝胶电泳 耐药性 

分 类 号：R516.5[医药卫生—内科学]