Cadmium induced a non-coding RNA microRNA535 mediates Cd accumulation in rice  

作　　者：Erkui Yue Fuxi Rong Zhen Liu Songlin Ruan Tao Lu Haifeng Qian 

机构地区：[1]College of Environment,Zhejiang University of Technology,Hangzhou 310032,China [2]Institute of Crop Science,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China [3]Institute of Crops,Hangzhou Academy of Agricultural Sciences,Hangzhou 310021,China [4]Hainan Institute,Zhejiang University,Hainan 572000,China

出　　处：《Journal of Environmental Sciences》2023年第8期149-162,共14页环境科学学报（英文版）

基　　金：This article supported by the Youth Program of National Natural Science Foundation of China(No.31901515);the National Natural Science Foundation of China(No.21976161).

摘　　要：Identifying key regulators related to cadmium(Cd)tolerance and accumulation is the main factor for genetic engineering to improve plants for bioremediation and ensure crop food safety.MicroRNAs(miRNAs),as fine-tuning regulators of genes,participate in various abiotic stress processes.MiR535 is an ancient conserved non-coding small RNA in land plants,positively responding to Cd stress.We investigated the effects of knocking out(mir535)and overexpressing miR535(mir535 and OE535)under Cd stress in rice plants in this study.The mir535 plants showed better Cd tolerance than wild type(WT),whereas the OE535 showed the opposite effect.Cd accumulated approximately 71.9%and 127%in the roots of mir535 and OE535 plants,respectively,compared to WT,after exposure to 2μmol/L Cd.In brown rice,the total Cd accumulation of OE535 and mir535 was about 78%greater and 35%lower than WT.When growing in 2 mg/kg Cd of soil,the Cd concentration was significantly lower in mir535 and higher in OE535 than in the WT;afterward,we further revealed the most possible target gene SQUAMOSA promoter binding-like transcription factor 7(SPL7)and it negatively regulates Nramp5 expression,which in turn regulates Cd metabolism.Therefore,the CRISPR/Cas9 technology may be a valuable strategy for creating new rice varieties to ensure food safety.

关 键 词：Cadmium stress MicroRNA535 CRISPR/Cas9 SQUAMOSA promoter binding-like transcription factors Food safety 

分 类 号：X503.231[环境科学与工程—环境工程] S511[农业科学—作物学]