酱香型酒糟中难挥发性化学成分的研究  

Identification of involatile chemical components from Moutai-flavored distiller’s grains

作　　者：林琳 齐晓冬 李永素 杨玉波 杨鸣华[2] 陈毅 孔令义[2] 王莉 LIN Lin;QI Xiaodong;LI Yongsu;YANG Yubo;YANG Minghua;CHEN Yi;KONG Lingyi;WANG Li(Kweichow Moutai Co.,Ltd.,Zunyi 564501;Laboratory of Natural Active Components and Pharmacodynamics Research,Pharmaceu-tical University,Nanjing 211198;Kweichow Moutai Group,Zunyi 564501,China)

机构地区：[1]贵州茅台酒股份有限公司,遵义564501 [2]中国药科大学天然活性组分与药效重点实验室,南京211198 [3]中国贵州茅台酒厂(集团)有限责任公司,遵义564501

出　　处：《中国药科大学学报》2023年第4期461-467,共7页Journal of China Pharmaceutical University

摘　　要：为了研究酱香型酒糟中难挥发性化学成分,实验以75%乙醇提取茅台酒糟,利用硅胶、ODS、Sephadex LH-20等柱色谱及制备型HPLC对酒糟石油醚、乙酸乙酯萃取部位进行分离纯化,得到21个化合物。化合物经ESI-MS和NMR波谱分析技术鉴定,分别鉴定为十五烷酸(1)、十六烷酸(2)、反式-2-癸烯酸(3)、十八酸正壬酯(4)、十八酸乙酯(5)、亚油酸乙酯(6)、十二烷酸(7)、1,3-二辛酰基-2-亚油酰基甘油(8)、环(苯丙氨酸-脯氨酸)(9)、环(脯氨酸-亮氨酸)(10)、3,6-双(2-甲基丙基)-2,5-哌嗪二酮(11)、4-羟基苯乙醇(12)、2,4-二羟基苯甲酸(13)、豆甾醇(14)、2-呋喃甲酸(15)、缬氨酸(16)、L-丙氨酰-L-脯氨酸(17)、二氢槲皮素(18)、5,7,3',4'-四羟基二氢黄酮(19)、槲皮素(20)、柚皮素(21)。化合物1~21为首次从酒糟中分离得到。In order to study the involatile chemical components in Moutai-flavored distiller’s grains,the Moutaiflavored distiller’s grains were extracted with 75%ethanol,followed by extraction with petroleum ether,ethyl acetate,and n-butanol.Silica gel,ODS,sephadex LH-20,and preparative HPLC were used to separate and iden⁃tify the petroleum ether and ethyl acetate layers.ESI-MS and NMR were used to identify the compounds,which were respectively identified as pentadecanoic acid(1),palmitic acid(2),trans-2-decenoic acid(3),n-nonyl octa⁃decanoate(4),ethyl octadecanoate(5),ethyl linoleate(6),luric acid(7),1,3-dicaprylyl-2-linoleylglycerin(8),cyclic(phenylalanine-proline)(9),cyclo-(proline-leucine)(10),3,6-bis-(2-methylpropyl)-2,5-dione piperazine(11),4-hydroxyphenethyl alcohol(12),2,4-dihydroxybenzoic acid(13),stigmasterol(14),2-furancarboxylic acid(15),valine(16),L-alanine acyl-L-proline(17),dihydroquercetin(18),5,7,3',4'-tetrahydroxyflavonoids(19),quercetin(20),and naringenin(21).Compounds 1-21 were isolated from distiller’s grains for the first time.

关 键 词：酱香型酒糟 石油醚部位 乙酸乙酯部位 分离纯化 结构鉴定 

分 类 号：R932[医药卫生—生药学]