二氢丹参酮Ⅰ对人肺癌A549和H1299细胞自噬的影响  

Effects of dihydrotanshinoneⅠon autophagy of human lung cancer A549 and H1299 cells

作　　者：杨钰基 代飞 何梦奇 孙岚 张珍珠[1] 张伟伟[1,2] 邵淑丽[1,2] YANG Yuji;DAI Fei;HE Mengqi;SUN Lan;ZHANG Zhenzhu;ZHANG Weiwei;SHAO Shuli(School of Life Sciences,Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《高师理科学刊》2023年第8期85-91,共7页Journal of Science of Teachers＇College and University

基　　金：黑龙江省高教强省优势特色学科——玉米“粮头食尾”重点项目(LTSW201737);黑龙江省省属高等学校基本科研业务费科研项目——植物性食品加工技术特色学科专项(YSTSXK201809);齐齐哈尔大学研究生创新科研项目(YJSCX2022022)。

摘　　要：以人肺癌A549和H1299细胞为研究对象,探究二氢丹参酮Ⅰ对其自噬的影响.采用qRT-PCR检测不同浓度二氢丹参酮Ⅰ作用人肺癌A549和H1299细胞48 h后自噬相关基因LC3,Beclin-1 mRNA的表达;采用Western Blot检测不同浓度二氢丹参酮Ⅰ作用人肺癌A549和H1299细胞48h后自噬相关基因LC3,Beclin-1蛋白的表达;免疫荧光法检测人肺癌A549和H1299细胞自噬泡.结果表明,经二氢丹参酮Ⅰ处理后,LC3和Beclin-1表达明显高于未加药处理组(P<0.05,P<0.01,P<0.001),LC3Ⅱ/LC3Ⅰ的比值增加(P<0.05,P<0.01,P<0.001);免疫荧光结果显示,经二氢丹参酮Ⅰ处理细胞后,细胞核周围出现集聚的绿色荧光,LC3蛋白集聚形成了自噬泡,表明一定浓度的二氢丹参酮Ⅰ可诱导人肺癌A549和H1299细胞发生自噬.Human lung cancer A549 and H1299 cells were used as research subjects to investigate the impact of dihydrotanshinone I on autophagy.qRT-PCR was employed to measure the expression of autophagy-related genes LC3 and Beclin-1 mRNA in A549 and H1299 cells after 48 hours of treatment with different concentrations of dihydrotanshinone I.Western Blot was used to detect the expression of autophagy related genes LC3 and Beclin-1 protein in human lung cancer A549 and H1299 cells treated with different concentrations of dihydrotanshinoneI for 48 h.Immunofluorescence was performed to detect autophagic vacuoles in A549 and H1299 cells.The results showed that after treatment with dihydrotanshinone I,the expression levels of LC3 and Beclin-1 were significantly higher than those in the untreated group(P<0.05,P<0.01,P<0.001),and the ratio of LC3Ⅱ/LC3Ⅰincreased(P<0.05,P<0.01,P<0.001).Immunofluorescence results revealed that upon dihydrotanshinone I treatment,green fluorescence accumulated around the nucleus,and LC3 protein aggregated to form autophagic vacuoles,indicating that a certain concentration of dihydrotanshinone I can induce autophagy in human lung cancer A549 and H1299 cells.

关 键 词：肺癌 二氢丹参酮Ⅰ 细胞自噬 

分 类 号：Q2[生物学—细胞生物学]