Ag负载螺旋碳纳米管修饰电极测定腺嘌呤  

Determination of Adenine Based on Ag-Loaded Helical Carbon Nanotubes Modified Electrode

作　　者：王帆 雷蕙疑 冯皓 旷文安 贺灵芝[3] WANG Fan;LEI Huiyi;FENG Hao;KUANG Wen'an;HE Lingzhi(College of Chemistry and Materials Science,Hengyang Normal University,Hengyang,Hunan 421008,China;The First Affiliated Hospital of Nanhua University,Hengyang,Hunan 421008,China;Hunan Polytechnic of Environmental Biology,Hengyang,Hunan 421008,China)

机构地区：[1]衡阳师范学院化学与材料科学学院,湖南衡阳421008 [2]南华大学附属第一医院,湖南衡阳421001 [3]湖南环境生物职业技术学院,湖南衡阳421005

出　　处：《浙江化工》2023年第8期46-53,共8页Zhejiang Chemical Industry

基　　金：湖南省卫生健康委科研课题(202211004542);衡阳师范学院创新平台开放基金项目(2021HSKFJJ022);湖南环境生物职业技术学院青年基金项目(ZK2020-01)。

摘　　要：腺嘌呤是核酸和辅酶的重要组成成分,腺嘌呤的缺失会导致生物体代谢功能的紊乱,开展生物体内腺嘌呤的检测有利于疾病预警。采用常规湿化学法合成了Ag负载螺旋碳纳米管(Ag/HMWCNTs),对腺嘌呤进行电化学定量检测。结果表明:与裸电极、螺旋多壁碳纳米管修饰电极相比,Ag/HMWCNTs修饰电极对腺嘌呤的氧化表现出较强的电催化性能。在最佳检测条件下,腺嘌呤浓度与其氧化峰电流在6.0×10^(-5)~8.0×10^(-3)mol/L有良好的线性关系,检出限低(9.0×10^(-6)mol/L),抗干扰强,可对实际样品进行检测。Adenine is an important component of nucleic acids and coenzymes,the loss of adenine will lead to the disorder of the metabolic function of the organism,and the detection of adenine in the organism is conducive to disease early warning.For the electrochemical quantitative detection of adenine,Ag-supported helical carbon nanotubes(Ag/HMWCNTs)were synthesized by conventional wet chemistry.The results show that compared with the bare electrode and spiral multi-walled carbon nanotube-modified electrode,Ag/HMWCNTs-modified electrode shows strong electrocatalytic performance for the oxidation of adenine.Under the best detection conditions,the adenine concentration has a good linear relationship with its oxidation peak current of 6.0×10^(-5)~8.0×10^(-3)mol/L,and the detection limit is low(9.0×10^(-6)mol/L),strong anti-interference,and can detect actual samples.

关 键 词：Ag负载螺旋碳纳米管 腺嘌呤 电化学检测 

分 类 号：R313[医药卫生—基础医学] O657.1[理学—分析化学]