抑制糖毒性通路对细胞线粒体功能障碍的影响和潜在意义  

The effect and implication of inhibiting glycotoxicity pathway on mitochondrial dysfunction

作　　者：李少莹 文莹 贾翠萍 张媛[1] 邓伟豪 Li Shaoying;Wen Ying;Jia Cuiping;Zhang Yuan;Deng Weihao(Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangzhou Guangdong 510623,China)

机构地区：[1]广州医科大学附属广州市妇女儿童医疗中心,广东广州510623

出　　处：《中华临床实验室管理电子杂志》2023年第2期65-70,共6页Chinese Journal of Clinical Laboratory Management(Electronic Edition）

基　　金：国家自然科学基金(82022033,81970437);广州市市校(院)联合资助项目(202201020651)。

摘　　要：目的探讨不同糖毒性抑制途径对细胞线粒体的影响,为糖尿病血管并发症提供新的治疗策略。方法去采用高糖刺激人脐静脉内皮细胞(HUVEC),分别在高糖刺激前后加入葡萄糖毒性途径抑制剂依帕司他、氮杂丝氨酸、氨基胍、索他霉素来抑制多元醇途径、已糖胺途径、晚期糖基化终末产物(AGE)和蛋白激酶C(PKC)通路。使用流式细胞仪分析测定HUVEC线粒体活性氧(ROS)水平,激光共聚焦显微镜观察线粒体分裂情况。结果采用高糖刺激,能明显升高HUVEC线粒体ROS水平及促进线粒体分裂;在高糖培养后,加入葡萄糖毒性途径抑制剂,细胞线粒体ROS水平和线粒体分裂无明显降低;然而在高葡萄糖刺激前,预先加入葡萄糖毒性途径抑制剂,能有效降低HUVEC中线粒体ROS水平和线粒体分裂情况。结论高糖刺激细胞线粒体分裂和ROS增加后,即使抑制糖毒性途径,也无法有效缓解细胞线粒体损伤。但是,当提前抑制糖毒性途径,细胞线粒体损伤可得到有效缓解。明确不同糖毒性抑制方式对细胞线粒体的影响,有望为糖尿病血管并发症的有效治疗提供更佳的治疗策略。Objective To explore the effects of various glycotoxicity pathway inhibitors on cellular mitochondria,and provide better therapeutic strategy for the effective treatment of diabetic vascular complications.Methods In this study,we inhibited polyol,hexosamine,AGE and PKC pathway with epalrestat,azaserine,aminoguanidine,sotrastaurin,respectively in human umbilical vein endothelial cell(HUVEC)before and after high glucose treatment.The mitochondria ROS was determined by flow cytometry and mitochondrial division was observed by confocal laser microscopy.Results The mitochondrial ROS and fragmentation of HUVEC were significantly increased by high glucose stimulation.After high glucose incubation,the mitochondrial ROS level and fragmentation did not decrease significantly.However,pre-addition of glucose-toxic pathway inhibitors before hyperglucose stimulation can effectively reduce mitochondrial ROS levels and mitochondrial division in HUVEC.Conclusions Inhibition of the glucotoxic pathway after high glucose stimulation cannot effectively alleviate mitochondrial damage.When the glycotoxic pathway is inhibited in advance,mitochondrial damage can be effectively alleviated.It is expected to provide better therapeutic strategies for the effective treatment of diabetic vascular complications by clarifying the effects of various glucotoxic pathway inhibitors on cellular mitochondria.

关 键 词：内皮细胞 活性氧 糖尿病 高血糖 

分 类 号：R285.5[医药卫生—中药学]