马齿苋多糖通过调控微小RNA-630的表达对宫颈癌细胞增殖和凋亡的影响  

Effects of Purslane polysaccharide on proliferation and apoptosis of cervical cancer cells by regulating microRNA-630 expression

作　　者：袁小波 阳帆 周丽丽 唐静 YUAN Xiao-bo;YANG Fan;ZHOU Li-li;TANG Jing(Medical Transformation Center,The First People's Hospital of Xiangtan,Xiangtan 411100,Hunan Province,China;Department of Nutrition,The First People's Hospital of Xiangtan,Xiangtan 411100,Hunan Province,China;School of Nursing,Hunan Institute of Communications Engineering,Xiangtan 411100,Hunan Province,China)

机构地区：[1]湘潭市第一人民医院,医学转化中心,湖南湘潭411100 [2]湘潭市第一人民医院,营养科,湖南湘潭411100 [3]湖南交通工程学院护理学院,湖南湘潭411100

出　　处：《中国临床药理学杂志》2023年第12期1718-1722,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的 探究马齿苋多糖调控微小RNA-630(miR-630)对宫颈癌细胞增殖和凋亡的影响。方法 将宫颈癌Caski细胞分为对照组(0μg·mL^(-1)的马齿苋多糖)、低剂量实验组(50μg·mL^(-1)马齿苋多糖)、中剂量实验组(100μg·mL^(-1)马齿苋多糖)、高剂量实验组(200μg·mL^(-1)马齿苋多糖)、anti-miR-NC组(转染anti-miR-NC)、anti-miR-630组(转染anti-miR-630)、miR-NC+PP组(转染anti-miR-NC+200μg·mL^(-1)马齿苋多糖)、miR-630+PP组(转染anti-miR-630+200μg·mL^(-1)马齿苋多糖)。以四甲基偶氮唑蓝(MTT)法检测细胞增殖;以流式细胞术检测细胞凋亡;以蛋白质印迹法检测细胞中细胞周期素D1(cyclin D1)、p21、B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)蛋白表达;以实时荧光定量聚合酶链反应(RT-qPCR)法检测细胞miR-630表达水平。结果 对照组、高剂量实验组miR-630表达水平分别为1.00±0.10和0.36±0.03;对照组、高剂量实验组、anti-miR-NC组、anti-miR-630组、miR-NC+PP组和miR-630+PP组细胞增殖抑制率分别为(0.00±0.01)%、(43.81±4.11)%、(6.78±0.33)%、(35.79±4.96)%、(37.98±4.11)%和(8.93±1.10)%;凋亡率分别为(5.66±0.15)%、(25.50±3.19)%、(7.95±0.46)%、(22.79±2.85)%、(26.98±2.97)%和(6.44±0.23)%;以上指标,高剂量实验组与对照组比较,anti-miR-NC组与anti-miR-630比较,miR-NC+PP组与miR-630+PP组比较,差异均有统计学意义(均P<0.05)。结论 马齿苋多糖通过抑制miR-630表达,促进宫颈癌细胞凋亡并抑制增殖。Objective To explore the effect of Purslane polysaccharide on the proliferation and apoptosis of cervical cancer cells by regulating microRNA-630(miR-630).Methods Cervical cancer Caski cells were divided into control group(Oμg·mL^(-1)Purslane polysaccharide),experimental-L group(50μg·mL^(-1) Purslane polysaccharide),experimental-M group(100μg·mL^(-1)Purslane polysaccharide),experimental-H group(200μg·mL^(-1) Purslane polysaccharide),anti-miR-NC group(transfected anti-miR-NC),anti-miR-630 group(transfected anti-miR-630),miR-NC+PP group(transfected anti-miR-NC+200μg·mL^(-1) Purslane polysaccharide)and miR-630+PP group(transfected with anti-miR-630+200μg·mL^(-1) Purslane polysaccharide).Cell proliferation was detected by methyl thiazolyl(MTT)method;cell apoptosis was detected by flow cytometry;cyclinD1,p21,B-cell lymphoma-2(Bcl-2)and Bcl-2 associated X protein(Bax)protein expressions in cells were detected by Western Blot;the expression level of cellular miR-630 was detected by quantitative real-time polymerase chain reaction(RT-qPCR).Results The levels of miR-630 in the control,experimental-H groups were 1.00±0.10,0.36±0.03,respectively.The inhibitory rates of cell proliferation in the control,experimental-H,anti-miR-NC,anti-miR-630,miR-NC+PP and miR-630+PP groups were(0.00±0.01)%,(43.81±4.11)%,(6.78±0.33)%,(35.79±4.96)%,(37.98±4.11)%。and(8.93±1.10)%,respectively;the apoptosis rates were(5.66±0.15)%,(25.50±3.19)%,(7.95±0.46)%,(22.79±2.85)%,(26.98±2.97)%and(6.44±0.23)%,respectively.For the above indexes,the experimental-H group were compared with the control group,the anti-miR-NC group was compared with the anti-miR-630 group,the miR-NC+PP group was compared with the miR-630+PP group,and the differences were statistically significant(all P<0.05).Conclusion Purslane polysaccharide can promote the apoptosis and inhibit the proliferation of cervical cancer cells by inhibiting the expression of miR-630.

关 键 词：马齿苋多糖 微小RNA-630 宫颈癌 增殖 凋亡 

分 类 号：R28[医药卫生—中药学]