黄芪总黄酮对大鼠急性心肌缺血再灌注损伤的保护作用研究  

Protective effect of total flavonoids from Astragali Radix on acute myocardial ischemia-reperfusion injury in rats

作　　者：唐艳艳 李飞泽 陈琳[1] 邵海斌 郝宗霞 李洁[1] TANG Yan-yan;LI Fei-ze;CHEN Lin;SHAO Hai-bin;HAO Zong-xia;LI Jie(Department of Cardiovascular Medicine,Zhoushan Hospital of Traditional Chinese Medicine,Affiliated to Zhejiang Universiyty of Traditional Chinese Medicine,Zhoushan 316000,Zhejiang Province,China)

机构地区：[1]浙江中医药大学附属舟山市中医院心血管科,浙江舟山316000

出　　处：《中国临床药理学杂志》2023年第12期1748-1752,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的 研究黄芪总黄酮对大鼠心肌缺血再灌注损伤的保护作用及其作用机制。方法 将大鼠左冠状动脉前降支进行结扎,使心肌缺血90 min,然后复通再灌注24 h,建立心肌缺血再灌注损伤模型。按随机分组法分成6组,每组10只。低、中、高剂量实验组分别给予50、150、300 mg·kg-^(1)TFA(以总黄酮量计算);复方丹参滴丸组给予146 mg·kg^(-1)复方丹参滴丸;假手术组和手术组给予等体积的0.9%NaCl,连续灌胃7 d,每天1次。记录大鼠心电图S-T段变化;心肌用氯化三苯基四氨唑(TTC)进行染色,观察心肌梗死面积,测定血清乳酸脱氢酶、肌酸激酶和心肌组织超氧化物歧化酶、丙二醛含量。结果 黄芪总黄酮在治疗7 d后对缺血再灌注损伤大鼠心电图S-T段有显著的修复作用。低、中、高剂量实验组、复方丹参滴丸组、手术组和假手术组心电图S-T段分别为(3.34±1.41)、(2.72±1.15)、(2.62±1.47)、(2.20±0.74)、(3.64±1.37)和(0.62±0.15)mV,中剂量实验组、高剂量实验组与手术组比较,S-T段均回落正常(均P<0.05)。低、中、高剂量实验组和复方丹参滴丸组、手术组和假手术组的心肌梗死面积分别为(15.00±3.40)%、(9.20±2.10)%、(7.35±1.80)%、(7.10±2.60)%、(21.60±3.70)%和0,与手术组相比,复方丹参滴丸组、低、中、高剂量实验组大鼠心肌梗死面积均降低,差异均有统计学意义(均P<0.05);中、高剂量实验组大鼠血清中乳酸脱氢酶、肌酸激酶显著降低;高剂量实验组减少大鼠心肌组织丙二醛的产生,提高心肌组织超氧化物歧化酶活力。结论 黄芪总黄酮可以降低心肌缺血再灌注损伤大鼠的心肌缺血程度,使心肌梗死面积减少,对缺血再灌注损伤具有一定的保护作用。Objective To investigate the protective effect of total flavonoids from Astragali Radix(TFA)on myocardial ischemia-reperfusion injury in rats and its mechanism.Methods The anterior descending branch of the left coronary artery of rats was ligated to bulid myocardial ischemia for 90 min,and then recanalized and reperfused for 24 h,so that the myocardial ischemia-reperfusion injury model was established.The rats were divided into 6 groups,10 rats in each group.Low,medium and high dose experimental groups were given 50,150,300 mg·kg^(-1)TFA(calculated by total flavonoids),respectively;compound Danshen dripping pills group was given 146 mg·kg^(-1)compound Danshen dripping pills;sham operation group and operation group were given equal volume of 0.9%NaCl.The rats were continuously given 7 d,once a day,by gavage.The S-T segment changes of electrocardiograph were observed and recorded in rats,and the myocardium was stained with triphenyltetrazolium chloride(TTC)to observe the area of myocardial infarction,the serum lactate dehydrogenase,creatine kinase and myocardial tissue superoxide dismutase and malondialdehyde content were determined.Results After 7 days of treatment,TFA had a significant repair effect on the S-T segment of the electrocardiogram in rats with ischemia reperfusion injury.The S-T segment of the electrocardiogram in low,medium,high dose experimental groups and compound Danshen dripping pills group,operation group,sham operation group were(3.34±1.41),(2.72±1.15),(2.62±1.47),(2.20±0.74),(3.64±1.37)and(0.62±0.15)mV,respectively.Compared with operation group,the S-T segment returned normally in medium and high dose experimental groups(all P<0.05).The myocardial infarction areas of low,medium,high dose experimental groups and compound Danshen dripping pills group,operation group,sham operation group were(15.00±3.40)%,(9.20±2.10)%,(7.35±1.80)%,(7.10±2.60)%,(21.60±3.70)%and 0,respectively.Compared with operation group,the myocardial infarction areas of compound Danshen dropping pills group and low

关 键 词：黄芪总黄酮 心肌缺血再灌注 超氧化物歧化酶 心肌梗死面积 心电图 

分 类 号：R28[医药卫生—中药学]