基于微流控芯片的细胞-细菌相互作用光电检测技术  

Photoelectric Detection Technologies of Cell-bacterial Interactions Based on The Microfluidic Chips

作　　者：谭浩兰 何红 龚丽[5] 葛闯 徐溢[1,3] TAN Hao-Lan;HE Hong;GONG Li;GE Chuang;XU Yi(Key Disciplines Lab of Novel Micro-Nano Devices and System Technology,Key Laboratory of Optoelectronic Technology and Systems,Ministry of Education,Chongqing University,Chongqing 400044,China;School of Chemistry and Chemical Engineering,Chongqing University,Chongqing 401331,China;School of Optoelectronic Engineering,Chongqing University,Chongqing 400044,China;Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment,Chongqing University Cancer Hospital,Chongqing 400030,China;School of Continuing Education,Chongqing Aerospace Polytechnic,Chongqing 400021,China)

机构地区：[1]重庆大学新型微纳米器件与系统技术重点学科实验室,光电技术与系统教育部重点实验室,重庆400044 [2]重庆大学化学化工学院,重庆401331 [3]重庆大学光电工程学院,重庆400044 [4]重庆大学附属肿瘤医院肿瘤转移与个体化诊治转化研究重庆市重点实验室,重庆400030 [5]重庆航天职业技术学院继续教育学院,重庆400021

出　　处：《生物化学与生物物理进展》2023年第6期1308-1318,共11页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(62071072);国家重点研发计划(2020YFB2009001);新型微纳器件与系统技术重点学科实验室开放基金(2020);智能传感与微纳生化系统(2019研究生导师团队)资助项目。

摘　　要：细胞/细菌及其相互作用研究对于生命科学、药物研发、医学诊疗等领域的研究具有重要意义。微流控芯片分析技术因微环境可控、生物相容性好、检测并行性、微型化等特性,正发展成为细胞/细菌及其相互作用研究的高效手段。本文在简要介绍基于微流控芯片分析技术的细胞-细菌分析方法和技术基础之上,对微流控芯片上细胞-细菌相互作用模型的建立进行了讨论,重点针对细胞-细菌及其相互作用过程的芯片检测进行了综述,尤其对芯片集成的光电检测技术及其测试效果进行总结和比较。通过芯片集成微流体控制、多种光电传感监测模块,使微流控芯片分析技术成为细胞/细菌及其相互作用过程分析和检测的支撑平台和优势手段。最后,对微流控光电检测技术在细胞-细菌相互作用检测中面临的挑战及发展趋势进行了讨论和展望。Cell-bacterial interactions refer to the process in which bacterium or bacterial metabolites act on the host cells,causing cellular and bacterial changes in morphology and functions.Cell-bacterial interactions researches are of vital significance to life sciences fields,such as drug development,disease diagnosis,and medical therapy.Recently,the detection and analysis of cell-bacterial interactions have developed rapidly.Cellular and bacterial morphology,activity,barrier function,and metabolites are the important clues to detect cell-bacterial interactions,which are essential to reveal deeper inflammatory disease mechanisms.Because of the controlled environment,good biocompatibility,multi-detection,and miniaturization,microfluidic analysis technology is developing into a powerful tool of cell-bacterial interaction research.In this review,we first introduce cell-bacterial analysis methods and technologies based on the microfluidic chip analysis briefly.Then the cell-bacterial interactions models based on the microfluidic chip are discussed,and later focusing on cell-bacterial interactions detection by microfluidic analysis technology,especially on the application of optical and electrochemical methods integrated with microfluidic chips.Microfluidic optical analysis system combines the chip with different microscopes,which is widely used for cellular and bacterial morphology imaging,and providing more details about metabolites with spectrometry during cell-bacterial interactions.Microfluidic electrochemical analysis system usually assemble directly microelectrodes on the chip,and its main advantage is monitoring dynamically cell-bacterial interaction through changes in electrical signals.By integrating microfluidics technologies and various detecting modules,microfluidic analysis technology has become an advantageous platform for cell-bacterial interactions analysis.Finally,the challenges and future development for microfluidic photoelectric detection technology in cell-bacterial interactions are discussed and prosp

关 键 词：细胞-细菌相互作用 细胞-细菌共培养 微流控芯片分析技术 光学检测法 电化学检测法 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] Q5-33[自动化与计算机技术—控制科学与工程] O65[生物学—生物化学]