基于分子印迹技术的细菌传感检测  

Molecular Imprinted Technology-based on Sensing Detection of Bacteria

作　　者：何勇飞 庞义全 葛闯 徐溢[1,3] HE Yong-Fei;PANG Yi-Quan;GE Chuang;XU Yi(Key Disciplines Lab of Novel Micro-Nano Devices and System Technology,Key Laboratory of Optoelectronic Technology and Systems,Ministry of Education,Chongqing University,Chongqing 400044,China;School of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400044,China;School of Optoelectronic Engineering,Chongqing University,Chongqing 400044,China;Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment,Chongqing University Cancer Hospital,Chongqing 400030,China)

机构地区：[1]重庆大学新型微纳器件与系统技术重点学科实验室&光电技术与系统教育部重点实验室,重庆400044 [2]重庆大学化学化工学院,重庆400044 [3]重庆大学光电工程学院,重庆400044 [4]重庆大学肿瘤医院肿瘤转移转化与个体化治疗重点实验室,重庆400030

出　　处：《生物化学与生物物理进展》2023年第6期1319-1329,共11页Progress In Biochemistry and Biophysics

基　　金：国家重点研发计划(2020YFB2009001);国家自然科学基金(62071072);中央高校基本科研业务费专项高校基金(2021CDJKYJH006);新型微纳器件与系统技术重点学科实验室开放基金(2020);智能传感与微纳生化系统(2019研究生导师团队)资助项目。

摘　　要：分子印迹因其材料结构的稳定性及靶标物识别的特异性而被广泛应用于生化分离分析的相关领域。近年来,将具有选择性捕获、分离和富集靶标物等优势的分子印迹技术与生化传感检测技术有机结合,是目前细菌等微生物高效检测领域备受关注的研究热点。本文就分子印迹技术在细菌分析中的印迹方法、分析检测技术和典型应用等方面的最新进展进行综述。首先介绍了细菌分子印迹原理,对表面印迹的材料以及直接压印、间接印迹和电聚合等制备方法进行了总结和归纳;重点对基于荧光、电化学、石英晶体微天平(QCM)等检测模式的细菌印迹传感监测在细菌分析检测及其与微流控芯片技术耦合的应用和进展进行了综述;最后,提出了存在的挑战及发展的趋势。Molecular imprinted technology has been applied to identify template molecules by using molecular imprinted polymers to simulate the interaction between enzymes and substrates or antibody and antigens.In recent years,to meet the needs of efficient microbial detection,bacteria,cells and other microbial imprinted technologies has been gradually derived from molecular imprinted technology,which was of the advantages of selective capture,effective separation and enrichment of targets,and easy to integrate sensing detection technology and so on.In this review,the latest advances of bacteria imprinted technology were introduced and summarized,including imprinting materials,imprinting mechanisms,detecting techniques and their typical applications.Firstly,the principle of bacteria imprinting was introduced.The materials of surface imprinting and the preparation methods of direct imprinting,indirect imprinting and electropolymerization were summarized.The applications and progress of bacteria imprinted sensing monitoring based on different analytical methods,such as fluorescence,electrochemistry,quartz crystal microbalance(QCM)and so no,were discussed in details.Especially,the analytical methodology based on microfluidic chip integrated with bacteria molecular imprinting was presented.Finally,the existing challenges of related fields and developing trends were put forward.It was illustrated that the imprinting technologies for bacteria,cells and other microbial could be widely adopted in the fields of biochemical separation and analysis because of its stability of material structure and specificity of target identification.

关 键 词：分子印迹技术 细菌 表面印迹 传感检测 微流控芯片 

分 类 号：Q599[生物学—生物化学] O65[理学—分析化学]