乙酰胆碱门控氯离子通道受体突变影响秀丽线虫运动学和运动状态转换  

Mutations in Acetylcholine-gated Chloride Channel Receptors Affect Locomotion Kinematics and Motor State Transitions in C. elegans

作　　者：玛伊拜尔·普拉提 熊荣康 霍菁 温泉 Mahiber Polat;XIONG Rong-Kang;HUO Jing;WEN Quan(Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230026,China;School of Data Science,University of Science and Technology of China,Hefei 230026,China;Hefei National Laboratory for Physical Sciences at the Microscale,Center for Integrative Imaging,University of Science and Technology of China,Hefei 230026,China;School of Basic Medical Sciences,Weifang Medical University,Weifang 261054,China)

机构地区：[1]中国科学技术大学生命科学与医学部,合肥230026 [2]中国科学技术大学大数据学院,合肥230026 [3]中国科学技术大学集成影像中心,合肥微尺度物质科学国家研究中心,合肥230026 [4]潍坊医学院基础医学院,潍坊261054

出　　处：《生物化学与生物物理进展》2023年第6期1381-1390,共10页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(32020103007)资助项目。

摘　　要：目的 乙酰胆碱作为一种高度保守的神经递质,在动物的运动行为调控中起着至关重要的作用。乙酰胆碱信号转导异常可导致多种运动功能障碍。然而,乙酰胆碱在运动行为中的抑制性调控机制尚未完全清楚。本文以秀丽隐杆线虫为研究对象,探究乙酰胆碱门控氯离子通道受体亚基(ACC-1、ACC-2、ACC-3、ACC-4)在运动行为中的调控作用。方法 通过将运动追踪、分子遗传学和光遗传学技术相结合,对乙酰胆碱门控氯离子通道受体亚基突变线虫的运动进行分析。结果 研究发现,这些亚基突变会影响线虫前进、后退和转向运动的运动学特征,并且前进过程中线虫身体弯曲幅度也发生了变化。在这些突变线虫的后退过程中光激活RIB中间神经元会导致后退运动延迟终止。结论 这些结果提示,乙酰胆碱门控氯离子通道亚基的调控作用对于维持和调节秀丽隐杆线虫运动状态是必需的。同时,这些亚基可能参与介导RIB中间神经元在秀丽隐杆线虫后退运动中的抑制性调控。本研究为理解乙酰胆碱门控抑制性受体在运动行为中的调控机制提供了新的思路。Objective Acetylcholine is a highly conserved neurotransmitter that plays a crucial role in the regulation of animal motor behavior.Abnormalities in acetylcholine signaling can lead to various motor dysfunctions.However,the inhibitory regulatory mechanisms of acetylcholine in motor behavior are not fully understood.In this study,we used Caenorhabditis elegans as a model organism to investigate the regulatory effects of acetylcholine-gated chloride channel receptor subunits(ACC-1,ACC-2,ACC-3,ACC-4)on motor behavior.Methods We used a combination of locomotion tracking,molecular genetics,and optogenetics to analyze C.elegans locomotion in acetylcholine-gated chloride channel subunit deficient mutants.Results We found that mutations in these subunits affected the kinematics of forward,backward,and turning movements of nematodes.The body bending amplitude during forward movement was also modified.Optogenetic activation of RIB interneurons led to delayed termination of the reversal in these mutant strains.Conclusion These results suggest that the regulation of acetylcholine-gated chloride channel subunits is required for maintaining and modulating C.elegans motor states.They also suggest that these subunits may be involved in mediating the inhibitory regulation of RIB interneurons on backward movement in C.elegans.This study provides new insights into the regulatory mechanisms of acetylcholine-gated inhibitory receptors in motor behavior.

关 键 词：秀丽隐杆线虫 乙酰胆碱门控氯离子通道受体 运动追踪 运动状态调控 

分 类 号：Q189[生物学—神经生物学]