大型褐藻类菌胞素氨基酸的活性分析和应用研究  

Activity Analysis and Application Study of Mycosporine-Like Amino Acids from Marine Brown Macroalgae

作　　者：赵秀芳 易俪慧 何阳迪 马骁 浦孟宣 朱文轩 王建莉[3] 孙颖颖 ZHAO Xiufang;YI Lihui;HE Yangdi;MA Xiao;PU Mengxuan;ZHU Wenxuan;WANG Jianli;SUN Yingying(Jiangsu Key Laboratory of Marine Bioresources and Eco-Environment,Jiangsu Ocean University,Lianyungang 222005,China;National Center of Quality Inspection&Testing on Deep Processing Silicon Products,Lianyungang 222300,China;State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;A Co-Innovation Center of Jiangsu Marine Bio-Industry Technology,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏连云港222005 [2]国家硅材料深加工产品质量检验检测中心,江苏连云港222300 [3]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [4]江苏省海洋生物产业技术协同创新中心,江苏海洋大学,江苏连云港222005

出　　处：《食品科技》2023年第6期202-209,共8页Food Science and Technology

基　　金：江苏省自然科学研究面上项目(BK20211353);江苏省优势学科项目;省科协系统南北共建项目;连云港市“海燕计划”项目(2020-QD-020);江苏海洋大学大学生创新训练计划项目。

摘　　要：类菌胞素氨基酸(Mycosporine-like amino acids,MAAs)是一类在食品、医药和化妆品等领域中具有广阔应用前景的活性化合物。羊栖菜、海带、马尾藻和裙带菜是MAAs的天然来源,然而,国内外未见此4种大型褐藻MAAs活性研究。为明确这些MAAs具有在食品中应用的潜力,文章首先分析了羊栖菜、海带、马尾藻和裙带菜MAAs提取物的体外抗氧化和吸湿保湿活性,并研究了它们对生湿面的保湿和对鲜切藕片的抗褐变作用。进一步,采用高效液相色谱-质谱方法测定4种大型褐藻MAAs组成。结果表明,羊栖菜等4种大型褐藻MAAs提取物具有一定的清除超氧阴离子、羟基自由基和DPPH自由基的能力,其中马尾藻MAAs提取物表现出最强的清除能力,对超氧阴离子、羟基自由基和DPPH自由基的半抑制率(IC50)依次为6.998、1.349 mg/mL和0.121 mg/mL。裙带菜和羊栖菜MAAs提取物的吸湿能力好于丙三醇和透明质酸,羊栖菜、马尾藻和裙带菜MAAs提取物保湿效果强于丙三醇和透明质酸。加入羊栖菜等4种大型褐藻MAAs提取物的鲜切藕片和生湿面在室温(15℃左右)放置4 d,样品的褐变指数和水分流失率均明显低于对照组。经高效液相色谱-质谱检测并与文献对比,确定了羊栖菜等4种大型褐藻MAAs提取物中58%~78%为Palythine、shinorine、porphyra-334和Palythenic acid等4种MAA,这可能是此4种大型褐藻MAAs提取物具有抗氧化和吸湿保湿活性的原因。羊栖菜等4种大型褐藻MAAs显示出在食品中的应用潜力。Mycosporine-like amino acids(MAAs)have broad application prospects in food,medicine,cosmetics and other fields.MAAs widely existed in brown macroalgae.Up to now,the research on the activity analysis of MAAs of brown macroalgae has not been reported at home and abroad.In order to clarify the potential of these MAAs in food applications,this work first analyzed the antioxidant,moisture absorption and moisturizing activities of MAA extracts from Hizikia fusifarme,Laminaria japonica,Sargassum sp.,and Undaria pinnatifida in vitro,and studied their moisturizing effects on wet noodles and anti-browning effects on fresh cut lotus root slices.Furthermore,the composition of MAAs from four species of brown macroalgae was determined by high performance liquid chromatography-mass spectrometry.Results showed that,MAA extracts from 4 species of brown macroalgae have some ability from scavenging superoxide anion,hydroxyl radical and DPPH radical.Among them,MAA extracts from Sargassum sp.showed the strongest scavenging ability,and the half inhibition rate(IC50)of superoxide anion,hydroxyl radical and DPPH radical was 6.998,1.349 mg/mL and 0.121 mg/mL,respectively.The moisture absorption capacity of MAA extracts from Undaria pinnatifida and Hizikia fusifarme is better than that of glycerol and hyaluronic acid,and the moisture retention effect of MAA extracts from Hizikia fusifarme,Sargassum sp.and Undaria pinnatifida is stronger than that of glycerol and hyaluronic acid.The fresh cut lotus root slices and wet noodles with MAA extracts from four species of brown macroalgae were placed at room temperature(about 15℃)for 4 days,and the browning index and water loss rate of these samples were significantly lower than those of the control group.After high performance liquid chromatography-mass spectrometry detection and comparison with the literature,58%~78%of these MAA extracts from the four species of brown macroalgae were identified as palythine,shinorine,porphyra-334 and palythenic acid,which may be the reason why the MAA extracts

关 键 词：类菌胞素氨基酸 羊栖菜 马尾藻 裙带菜 吸湿保湿活性 抗褐变作用 

分 类 号：R284.2[医药卫生—中药学]