药物保留灌肠对放射性直肠炎大鼠的药效学研究  

Pharmacodynamic Study of Drug Retention Enema in Rats with Radiation Proctitis

作　　者：李雅菲 汪代杰[1] 张燕 文庆莲 LI Yafei;WANG Daijie;ZHANG Yan;WEN Qinglian(Luzhou People's Hospital,Luzhou Sichuan 646000,China;The Affiliated Hospital of Southwest Medical University,Luzhou Sichuan 646000,China)

机构地区：[1]泸州市人民医院,四川泸州646000 [2]西南医科大学附属医院,四川泸州646000

出　　处：《中医药导报》2023年第8期8-12,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：泸州市科技计划项目[2016-S-67(13/23)]。

摘　　要：目的:探究消痔灵、谷氨酰胺、美沙拉嗪、庆大霉素混合保留灌肠治疗放射性直肠炎的安全性及有效性。方法:将50只SD大鼠随机分为对照组、模型组、低剂量组、中剂量组、高剂量组,每组10只。对照组大鼠常规饲养,模型组大鼠构建放射性直肠炎模型;低、中、高剂量组大鼠在构建放射性直肠炎大鼠模型的基础上用不同剂量的药物混合保留灌肠,对照组、模型组大鼠采用生理盐水灌肠。14 d后观察大鼠一般体征并记录相应的评分;检测血常规和血生化指标;HE染色观察直肠组织病理学变化并进行分级;ELISA检测血清白介素-6(IL-6)、白介素-8(IL-8)和肿瘤坏死因子-α(TNF-α)含量。结果:模型组大鼠一般体征评分、病理损伤评分、红细胞计数(RBC)、血红蛋白(HGB)、红细胞压积(HCT)、总蛋白(T-PROT)、白蛋白(ALB)及血清中IL-6、IL-8、TNF-α含量均高于对照组,白细胞计数(WBC)低于对照组(P<0.05)。低、中、高剂量组大鼠一般体征评分、病理损伤评分、RBC、HGB、HCT、T-PROT、ALB及血清中IL-6、IL-8和TNF-α含量均低于模型组,WBC高于模型组(P<0.05),且随着保留灌肠药物剂量的升高,效果越好(P<0.05)。结论:消痔灵、谷氨酰胺、美沙拉嗪、庆大霉保留灌肠治疗放射性直肠炎,安全有效,且以高剂量最为合适。Objective:To explore the safety and efficacy of Xiaozhiling(消痔灵),glutamine,mesalazine and gentamicin mixed retention enema in the treatment of radiation proctitis.Methods:A total of 50 SD rats were randomly divided into control group,model group,low-dose group,medium-dose group and high-dose group,with 10 rats in each group.The rats in the control group were fed routinely,and the rats in the model group were constructed with radiation proctitis model.On the basis of constructing the rat model of radiation proctitis,the rats in the low,medium and high-dose groups were mixed with different doses of drugs for retention enema,and the rats in the control group and the model group were enema with normal saline.After 14 days,the general signs of rats were observed and the corresponding scores were recorded.The blood routine and blood biochemical indexes were detected.The pathological changes of rectal tissue were observed and graded by HE staining.The levels of serum interleukin-6(IL-6),interleukin-8(IL-8)and tumor necrosis factor-α(TNF-α)were detected by ELISA.Results:The general physical signs and pathological injury score,red blood cell count(RBC),hemoglobin(HGB),hematocrit(HCT)total protein(T-PROT)and albumin(ALB)and serum IL-6,IL-8 and TNF-αin the model group were higher than those in control group,and the white blood cell count(WBC)was lower than that in control group(P<0.05).The scores of general signs and pathological injury score,RBC,HGB,HCT,T-PROT,ALB and serum expressions of IL-6,IL-8 and TNF-αin low-dose group,medium-dose group and high-dose group were lower than those in model group,and WBC was higher than that in model group(P<0.05),and the effect was better with the increase of drug retention enema dose(P<0.05).Conclusion:Retention enema with Xiaozhiling(消痔灵),glutamine,mesalazine and gentamicin is safe and effective for radiation proctitis,and high dose is the most suitable.

关 键 词：放射性直肠炎 药物保留灌肠 消痔灵 谷氨酰胺 美沙拉嗪 庆大霉素 药效学研究 大鼠 

分 类 号：R285.5[医药卫生—中药学]