重离子径迹蚀刻膜处理甘蔗废糖蜜提升酪丁酸梭菌代谢丁酸效能研究  

Improving Butyric Acid Metabolic Effectiveness of Clostridium Tyrobutyricum in Waste Sugarcane Molasses by Heavy Ion Track Etching Membrane

作　　者：王冰 王娅娟 周翔[2,3] 陆栋 李欣[1] WANG Bing;WANG Yajuan;ZHOU Xiang;LU Dong;LI Xin(College of Food and Bioengineering,Henan University of science and technology,Luoyang 471000,Henan,China;Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China;University of Chinese Academy of Sciences,Chinese Academy of Science,Beijing 100049,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471000 [2]中国科学院近代物理研究所,兰州730000 [3]中国科学院大学,北京100049

出　　处：《原子核物理评论》2023年第2期294-302,共9页Nuclear Physics Review

基　　金：河南省自然科学基金资助项目(182300410083);中国科学院STS项目(KFJ-STS-QYZD-197)。

摘　　要：为对工业废料甘蔗废糖蜜进行资源化回收利用,选择绿色膜处理工艺——重离子径迹蚀刻膜,对预处理的甘蔗废糖蜜进行过滤,并用于酪丁酸梭菌合成丁酸。采用激光粒度分析仪和超高效液相色谱-串联质谱法检测甘蔗废糖蜜中的粒径分布和多酚类物质的含量,结果表明,糖蜜中的胶体、灰分和多酚类等毒性物质可被蚀刻膜过滤。碘化丙啶染色法的结果表明,在膜处理后的甘蔗废糖蜜中,酪丁酸梭菌的存活率高达98.30%。经气相色谱-质谱法检测,以膜处理后的甘蔗废糖蜜发酵生产丁酸,丁酸的产量为39.08 g/L。重离子径迹蚀刻膜结合化学法将甘蔗废糖蜜变废为宝,无污染、零排放,科学有效地提高了酪丁酸梭菌对甘蔗废糖蜜的利用效能及产丁酸性能。In order to recycle and utilize industrial waste sugarcane molasses,a green membrane treatment technology:heavy ion track etching membrane was selected to filter the pretreated waste sugarcane molasses and then used to synthesize butyric acid by Clostridium tyrobutyricum(C.tyrobutyricum).The particle size distribution and polyphenol content of waste sugarcane molasses were determined by Laser Particle Size Analyzer and Ultra Performance Liquid Chromatography—Tandem Mass Spectrometry.Results showed that the toxic substances such as colloid,ash and polyphenols in molasses can be filtered by etching membrane.The staining results of Propidium Iodide indicated that the survival rate of C.tyrobutyricum in the waste sugarcane molasses treated with membrane was as high as 98.30%.The yield of butyric acid produced from waste sugarcane molasses after membrane treatment was 39.08 g/L,which was detected by Gas Chromatography—Mass Spectrometer.Waste sugarcane molasses was successfully transformed from waste to treasure by heavy ion track etching membrane combined with chemical method,with no pollution and zero emission during whole process.It improved the utilization efficiency of waste sugarcane molasses and the production performance of butyric acid by C.tyrobutyricum scientifically and effectively.

关 键 词：重离子径迹蚀刻膜 甘蔗废糖蜜 多酚类物质 酪丁酸梭菌 丁酸 

分 类 号：Q815[生物学—生物工程]