2017-2021年贵州省布鲁氏菌分离株的多位点序列分型基因特征  

Genetic characteristics of multi-locus sequence typing of Brucella isolates in Guizhou Province from 2017 to 2021

作　　者：王月[1] 谭勤琴 刘英[1] 杨幸贵 营夏 马青[1] 韦小瑜[1] 李世军[1] Wang Yue;Tan Qinqin;Liu Ying;Yang Xinggui;Ying Xia;Ma Qing;Wei Xiaoyu;Li Shijun(Department of Bacterial Testing,Guizhou Center for Disease Control and Prevention,Guiyang 550004,China;Key Laboratory for Environmental Pollution Monitor and Disease Control,Ministry of Education,School of Public Health,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]贵州省疾病预防控制中心细菌性检验科,贵阳550004 [2]贵州医科大学公共卫生与健康学院,环境污染与疾病监控教育部重点实验室,贵阳550025

出　　处：《中华地方病学杂志》2023年第5期351-355,共5页Chinese Journal of Endemiology

基　　金：贵州省传染病人才培养基地建设项目子项目(RCJD2102)。

摘　　要：目的了解贵州省布鲁氏菌分离株的多位点序列分型(multi-locus sequence typing,MLST)基因特征。方法以2017-2021年贵州省布鲁氏菌分离株(保存于贵州省疾病预防控制中心菌毒种库)为研究对象,应用BCSP31-PCR方法进行布鲁氏菌属特异性鉴定,AMOS-PCR方法进行布鲁氏菌种/型鉴定;应用MLST方法进行基因分型,并采用Bionumerics 8.0软件对分型结果进行聚类分析。结果贵州省分离的32株布鲁氏菌,经BCSP31-PCR方法和AMOS-PCR方法鉴定为羊种布鲁氏菌;经MLST方法分析,可以分为2种ST型(ST8、ST39型),其中ST8型28株(87.5%)、ST39型4株(12.5%)。ST8型菌株分布在省内7个市(州),ST39型菌株仅在黔西南布依族苗族自治州发现。聚类分析结果显示,ST8、ST39型菌株与羊种布鲁氏菌参考株聚在1个群内,且ST39型与ST8型菌株仅在glk基因内存在1个核苷酸位点差异,亲缘关系较近。结论羊种布鲁氏菌是近年来贵州省布鲁氏菌病疫情的主要病原体,ST8型是贵州省羊种布鲁氏菌的优势MLST基因型。Objective To study the multi-locus sequence typing(MLST)gene characteristics of Brucella isolates in Guizhou Province.Methods Brucella strains,which were isolated from 2017 to 2021 in Guizhou Province(preserved in the Bacterial and Viral Seed Bank of Guizhou Center for Disease Control and Prevention)were identified Brucella and species/types by BCSP31-PCR and AMOS-PCR methods,respectively.MLST method was used for genotyping,and Biometrics 8.0 software was used for cluster analysis of the typing results.Results A total of 32 strains of Brucella were isolated in Guizhou Province and identified as Brucella melitensis(B.melitensis)by BCSP31-PCR and AMOS-PCR methods.These strains were classified into 2 ST types(ST8 and ST39)by MLST method,with 28 strains of ST8 type(87.5%)and 4 strains of ST39 type(12.5%).The 28 strains of ST8 type were distributed in 7 cities(prefectures)of Guizhou Province,while the 4 strains of ST39 type were only found in Qianxinan Buyi and Miao Autonomous Prefecture.The cluster analysis results showed that ST8 and ST39 types strains were clustered in a group with the reference strain of B.melitensis,and there was only one nucleotide site difference between ST39 and ST8 types in the glk gene,indicating a close genetic relationship.Conclusions B.melitensis is the main pathogen of the brucellosis epidemic in Guizhou Province in recent years.ST8 is the dominant MLST genotype in Guizhou Province.

关 键 词：布鲁杆菌属 多位点序列分型 分布 

分 类 号：R378.5[医药卫生—病原生物学]