Correlation Analysis of Molecularly-Defined Cortical Interneuron Populations with Morpho-Electric Properties in Layer V of Mouse Neocortex  

作　　者：Jun-Wei Cao Xiao-Yi Mao Liang Zhu Zhi-Shuo Zhou Shao-Na Jiang Lin-Yun Liu Shu-Qing Zhang Yinghui Fu Wen-Dong Xu Yong-Chun Yu 

机构地区：[1]Jing’an District Central Hospital of Shanghai,State Key Laboratory of Medical Neurobiology,MOE Frontiers Center for Brain Science,Institutes of Brain Science,Fudan University,Shanghai 200032,China [2]School of Life Sciences,Fudan University,Shanghai 200433,China [3]Department of Vascular Surgery,Shanghai Pudong Hospital,Fudan University Pudong Medical Center,Shanghai 201399,China [4]School of Data Science,Fudan University,Shanghai 200433,China [5]The National Clinical Research Center for Aging and Medicine,Huashan Hospital,Fudan University,Shanghai 200032,China

出　　处：《Neuroscience Bulletin》2023年第7期1069-1086,共18页神经科学通报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2021ZD0202500);supported by the National Natural Science Foundation of China(31930044 and 31725012);the Foundation of Shanghai Municipal Education Commission(2019-01-07-00-07-E00062);the Collaborative Innovation Program of Shanghai Municipal Health Commission(2020CXJQ01);the Shanghai Municipal Science and Technology Major Project(No.2018SHZDZX01);ZJLab.

摘　　要：Cortical interneurons can be categorized into distinct populations based on multiple modalities,including molecular signatures and morpho-electrical(M/E)properties.Recently,many transcriptomic signatures based on single-cell RNA-seq have been identified in cortical interneurons.However,whether different interneuron populations defined by transcriptomic signature expressions correspond to distinct M/E subtypes is still unknown.Here,we applied the Patch-PCR approach to simultaneously obtain the M/E properties and messenger RNA(mRNA)expression of>600 interneurons in layer V of the mouse somatosensory cortex(S1).Subsequently,we identified 11 M/E subtypes,9 neurochemical cell populations(NCs),and 20 transcriptomic cell populations(TCs)in this cortical lamina.Further analysis revealed that cells in many NCs and TCs comprised several M/E types and were difficult to clearly distinguish morpho-electrically.A similar analysis of layer V interneurons of mouse primary visual cortex(V1)and motor cortex(M1)gave results largely comparable to S1.Comparison between S1,V1,and M1 suggested that,compared to V1,S1 interneurons were morpho-electrically more similar to M1.Our study reveals the presence of substantial M/E variations in cortical interneuron populations defined by molecular expression.

关 键 词：Cortical interneuron Cell population Transcriptomic signature Morpho-electrical properties Correlation analysis Brain area 

分 类 号：R338[医药卫生—人体生理学]