炭基材料固定化真菌漆酶在污水处理领域的应用  

Application of immobilized fungal laccase on carbonaceous materials for wastewater treatment

作　　者：徐乐天 王旺民 付龙汶 孙展飞 高域洲 储刚 孙凯[1] 司友斌[1] XU Letian;WANG Wangmin;FU Longwen;SUN Zhanfei;GAO Yuzhou;CHU Gang;SUN Kai;SI Youbin(School of resources and environment,Anhui Agricultural University,Hefei,230036,China)

机构地区：[1]安徽农业大学资源与环境学院,合肥230036

出　　处：《环境化学》2023年第8期2823-2833,共11页Environmental Chemistry

基　　金：国家自然科学基金(42107406);安徽省自然科学基金(2008085QD186);安徽省高校自然科学研究项目(KJ2019A0204)资助.

摘　　要：漆酶是一种多功能含铜氧化还原酶,被广泛研究或应用于多个学科领域.然而,实际应用中,漆酶因成本昂贵、易流失,稳定性不足等缺陷,限制了其工业化应用和商业化价值.炭基材料固定化真菌漆酶技术提供了一种值得探索的途径,来改善真菌漆酶的贮存稳定性、催化氧化活性、以及重复使用的性能.本文详细介绍了真菌漆酶的来源、结构和性质;系统归纳了生物炭、活性炭、碳纳米管、石墨烯等炭基材料固定化真菌漆酶的物理-化学方法(物理吸附、诱捕包埋、共价结合、交联聚合);概述了酚类、雌激素、染料、药物等环境有机污染物在炭基固定化真菌漆酶复合材料上的去除效果和循环性能,并深入讨论了化合物在炭质界面的吸附机制(如疏水性作用、π-π作用、孔隙填充等)和固定化真菌漆酶利用氧化还原电势差催化氧化有机物的反应机制.本文旨在为真菌漆酶固定化载体材料的选择提供参考依据,也将为开拓固定化真菌漆酶应用于有机污染治理的视野提供技术支撑.Laccase is one of multi-copper containing oxidoreductases,which has been widely studied or applied in multiple subject fields.However,the industrial application and commercial value of laccase is limited in practical production due to expensive cost,difficult recycling,insufficient stability.The immobilization of fungal laccase by carbonaceous materials provides an approach to explore to improve the storage stability,catalytic oxidation activity,and reusability.This work described the origin,structure and properties of fungal laccase,and systematically concluded the physico-chemical methods including physical adsorption,trapping and embedding,covalent binding,crosslinking.Meanwhile,a series of carbonaceous materials such as biochar,activated carbon,carbon nanotubes,graphene was summarized to immobilize the fungal laccase.The removal of organic contaminants(phenols,estrogen,dyes,and drugs)and recycling performance of immobilized laccase were outlined and its reactive mechanisms including sorption and catalytic oxidation were deeply discussed between immobilized laccase and environmental contaminants.This article aims at providing an insight to select the carrier materials to the immobilization of fungal laccase,and also providing a technical support for expanding the application scope of immobilized laccase in organic pollution control.

关 键 词：炭基材料 真菌漆酶 固定方法 吸附降解 循环利用 

分 类 号：X703[环境科学与工程—环境工程]