时珍法物种鉴定原理与研究策略  

Principles and strategies for species identification based on analysis of whole-genome

作　　者：甘雨桐 辛天怡 许文杰 郝利军 齐桂红 娄千 宋经元 GAN Yu-tong;XIN Tian-yi;XU Wen-jie;HAO Li-jun;QI Gui-hong;LOU Qian;SONG Jing-yuan(Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People's Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;Engineering Research Center of Chinese Medicine Resource,Ministry of Education,Beijing 100193,China;Yunnan Branch,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Jinghong 666100,China)

机构地区：[1]中国医学科学院、北京协和医学院药用植物研究所,国家中医药管理局中药资源保护重点研究室,北京100193 [2]中药资源教育部工程研究中心,北京100193 [3]中国医学科学院、北京协和医学院药用植物研究所云南分所,云南景洪666100

出　　处：《药学学报》2023年第8期2364-2374,共11页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81874339);中国医学科学院医学与健康科技创新工程(2022-I2M-2-001).

摘　　要：天然药物的主要来源包括植物、动物和微生物等生物物种。这些物种的准确鉴定是天然药物研发的基础。本文提出一种全新的物种鉴定方法:时珍法(analysis of whole-genome,AGE),即从物种全基因组寻找特异靶标序列,精准识别特异靶标序列,实现物种准确鉴定的分子诊断方法。笔者详细阐述了时珍法利用不同物种基因组序列必然存在差异的原理实施物种鉴定,提出该方法的实施策略可分为研究和应用两个层面,分析其特点评判该方法具有原理可靠、特异性强、适用性广等优势,进一步论述了在体系构建过程涉及的基因组获取、生物信息分析、数据库构建等三个关键问题。综上所述,本文为生物信息软件和商品试剂盒的后续开发提供理论基础和方法指导,表明时珍法在不同对象、不同学科、不同行业等方面具有巨大应用潜力。The main sources of natural drugs include various biological species such as plants,animals,and microorganisms.The accurate identification of these species is the bedrock of natural drug development.We propose a novel method of species identification in this paper:analysis of whole-genome(AGE),a molecular diagnostic method used to identify species by finding species-specific sequences from the whole genome and precisely recognizing the specific target sequences.We elaborate that the principle for species identification based on AGE is that the genome sequences of diverse species must differ and divide the implementation strategy of the method into two levels of research and application.Based on our analysis of its characteristics,the method would have the potential advantages of reliable principle,high specificity,and wide applicability.Moreover,three crucial concerns related to building method systems including genome acquisition,bioinformatics analysis,and database construction,are further discussed.In summary,we offer theoretical underpinnings and methodological guidance for the development of bioinformatics software and commercial kits,indicating AGE has great application potential in objects,subjects,and industries.

关 键 词：时珍法 物种鉴定 全基因组 生物信息分析 数据库构建 

分 类 号：R932[医药卫生—生药学]