基于全基因组的栀子苷生物合成相关MYB转录因子系统分析  

Systematic analysis of MYB transcription factors related to the geniposide biosynthesis in Gardenia jasminoides Ellis based on whole genome

作　　者：许文杰 黄远浩 韩蓉蓉 刘燕琴[2] 曹敏[2] 宋经元[1,3] XU Wen-jie;HUANG Yuan-hao;HAN Rong-rong;LIU Yan-qin;CAO Min;SONG Jing-yuan(Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People's Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;Chongqing Institute of Medicinal Plant Cultivation,Chongqing 408435,China;Engineering Research Center of Chinese Medicine Resource,Ministry of Education,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,国家中医药管理局中药资源保护重点研究室,北京100193 [2]重庆市药物种植研究所,重庆408435 [3]中药资源教育部工程研究中心,北京100193

出　　处：《药学学报》2023年第8期2522-2531,共10页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金项目(82073966);川渝道地药材绿色种植技术研究与应用(cstc2020jscx-cylhX0008).

摘　　要：MYB转录因子参与调控多种次生代谢产物的生物合成。栀子是我国大宗常用中药材,主要活性成分为栀子苷。本研究以栀子不同发育时期的叶片和花蕾器官为研究材料,借助基因组和转录组分析,全面挖掘栀子苷生物合成相关MYB转录因子。转录组数据分析表明,不同于萜类合成共有途径基因表达模式,栀子苷合成特异途径基因在花蕾中的表达量显著高于叶片,表达模式与该成分的器官积累规律相同,且香叶醇合酶、环烯醚萜合酶和栀子苷酸甲基转移酶编码基因的启动子区域均含有多个MYB转录因子结合位点。对栀子基因组编码基因进行注释,共获得105个MYB转录因子,根据保守结构域数量将其划分为68个1R-MYB、33个R2R3-MYB、3个3R-MYB和1个非典型MYB转录因子。进一步结合系统发育树和实时荧光定量PCR分析,筛选出3个可能调控栀子苷生物合成的候选MYB转录因子,包括潜在正调控转录因子GjMYB23及负调控转录因子GjMYB31和GjMYB73。本研究结果将为栀子苷生物合成调控研究及进一步解析栀子品质形成机制奠定基础,以推动栀子优良品种选育。MYB transcription factors are involved in the regulation of various secondary metabolites biosynthesis.Gardenia jasminoides Ellis is the commonly used Chinese herbal medicine,and its main active ingredient is geniposide.Here,leaves and flower buds at different developmental stages of G.jasminoides were used to explore MYB transcription factors related to geniposide biosynthesis based on genome and transcriptome analysis.Transcriptome data analysis showed that,different from the expression of the common pathway genes for terpenoid biosynthesis,the expression level of genes in the specific pathway of geniposide biosynthesis was significantly higher in flower buds than in leaves,which was the same as the organ accumulation pattern of this component.And the promoter regions of geraniol synthase,iridoid synthase and geniposidic acid methyltransferase involved in the specific pathway all contained multiple MYB-binding sites.A total of 105 MYB transcription factors were obtained by annotating the coding genes of G.jasminoides,which were divided into 681R-MYB,33 R2R3-MYB,33R-MYB and 1 atypical MYB transcription factor according to the number of conserved domain.Based on the analysis of phylogenetic tree and quantitative real-time PCR,three candidate MYB transcription factors related to geniposide biosynthesis were selected,including potential positive regulation factor GjMYB23 and negative regulation factors GjMYB31 and GjMYB73.The results of this study will lay a foundation for searching the regulation of geniposide biosynthesis and further analysis of the quality formation mechanism of G.jasminoides,so as to promote the breeding of excellent varieties of G.jasminoides.

关 键 词：栀子 栀子苷 MYB 转录因子 基因组 

分 类 号：R932[医药卫生—生药学]