The quantity of OA and activity of cellulase and polygalacturonase are involved in variation of virulence in Sclerotium rolfsii  

作　　者：Dongyang Yu Wanduo Song Qianqian Wang Yanping Kang Yong Lei Zhihui Wang Yuning Chen Dongxin Huai Wang Xin Boshou Liao Liying Yan 

机构地区：[1]Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agricultural and Rural Affairs,Hubei,Wuhan,430062,China

出　　处：《Oil Crop Science》2023年第2期121-126,共6页中国油料作物学报(英文版）

基　　金：supported by Central Public-interest Scientific Institution Basal Research Fund (1610172021003);Supported by the earmarked fund for CARS-13;Key Area Research and Development Program of Hubei Province (2021BBA077)

摘　　要：In order to understand the pathogenic mechanisms of Sclerotium rolfsii on peanut and to analyze the variation of virulence in S.rolfsii strains,the highly virulent strain(ZY2)and weakly virulent strain(GP3-1)were investigated under both in vivo and in vitro conditions.The results indicated that S.rolfsii directly infected peanut by producing infection cushions.ZY2 formed infection cushions earlier than GP3-1,and ZY2 produced a greater number of infection cushions compare to GP3-1.Both strains could utilize cellulose,xylose,or polygalacturonic acid in the Czapek medium.The activities of cellulase(CL)and polygalacturonase(PG)in the inoculated peanut stems increased significantly at 9 h after inoculation.The activities of CL and PG produced by ZY2 in the inoculated stems were significantly higher than that produced by GP3-1.Both strains could produce oxalic acid(OA),and the content of OA produced by ZY2 in the inoculated stems was higher than that produced by GP3-1.In summary,it suggested that S.rolfsii destroyed peanut cells through physical and biochemical factors by secreting a large amount of OA,CL and PG during the formation of infection cushions.The difference in OA content,activity of CL and PG produced by highly and weakly virulent strains played important roles in variation of virulence.

关 键 词：Sclerotium rolfsii Sacc. PATHOGENICITY Virulence variation Microscopic observation Oxalic acid Cell wall degrading enzyme 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]