两种盐生植物内生真菌多样性及其功能预测  

Diversity and roles of endophytic fungi in two halophytes

作　　者：高磊[1,2] 黄银 马金彪 蒋宏忱[1,3] 李文均 李丽[1] GAO Lei;HUANG Yin;MA Jinbiao;JIANG Hongchen;LI Wenjun;LI Li(State Key Laboratory of Desert and Oasis Ecology,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,Xinjiang,China;University of Chinese Academy of Sciences,Beijing 100049,China;State Key Laboratory of Biogeology and Environmental Geology,China University of Geosciences,Wuhan 430074,Hubei,China;State Key Laboratory of Pest Biocontrol and Resource Utilization,School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,Guangdong,China)

机构地区：[1]中国科学院新疆生态与地理研究所荒漠与绿洲生态国家重点实验室,新疆乌鲁木齐830011 [2]中国科学院大学,北京100049 [3]中国地质大学(武汉)生物地质与环境地质国家重点实验室,湖北武汉430074 [4]中山大学生命科学学院有害生物控制与资源利用国家重点实验室,广东广州510275

出　　处：《微生物学通报》2023年第8期3357-3371,共15页Microbiology China

基　　金：新疆维吾尔自治区上海合作组织科技伙伴计划(2020E01047)。

摘　　要：【背景】植物内生菌是微生物群落中一类非常重要的组成部分,是重要的微生物资源库,在植物促生、抗逆等多个领域有重要的研究和应用价值。【目的】进一步了解干旱荒漠区盐生植物内生真菌的多样性、群落结构和潜在功能特征。【方法】对生长在乌兹别克斯坦西咸海岸边的两种盐生植物毛足假木贼(Anabasis eriopoda abbreviated as AE)和展枝假木贼(A.truncata abbreviated as AT)的内生真菌群落进行扩增子测序分析。【结果】共获得166个amplicon sequencing variants(ASVs),涉及4门49属,其中Neocamarosporium、Botryosphaeria和Alternaria及其所属高级分类单元是优势类群。多样性和群落组成分析显示两种盐生植物的内生真菌存在较为明显的差异,并包含一些潜在的新分类单元。基于PICRUSt2和FUNGuild的功能预测结果表明这两种盐生植物内生真菌的潜在功能和营养方式多样且表现出宿主差异性。【结论】盐生植物内生真菌具有较高的多样性和潜在的资源价值,有待进一步挖掘和研究。[Background]Endophytes associated with halophytes have a wide range of activities,such as promoting plant growth,improving stress resistance,fixing nitrogen,and degrading toxic compounds.[Objective]To deeply understand the diversity,community structure,and potential roles of endophytic fungi of halophytes in the arid land.[Methods]Amplicon sequencing was performed for the endophytic fungi in two halophytes(Anabasis eriopoda and A.truncata)growing on the coast of the western Aral Sea in Uzbekistan.[Results]A total of 166 amplicon sequencing variants(ASVs)were obtained,representing 49 genera of 4 phyla,among which the dominant taxa were Neocamarosporium,Botryosphaeria,Alternaria and their higher taxa.The diversity and community composition of endophytic fungi showed significant differences between the two halophytes,which harbored potential novel taxa.Functional prediction by PICRUSt2 and FUNGuild demonstrated that the endophytic fungi in the two halophytes exhibited diverse roles and trophic modes,being host specific.[Conclusion]Our results suggest that the endophytic fungi in halophytes have high diversity and potential resource values and remain to be studied.

关 键 词：干旱区 盐生植物 内生真菌 多样性 功能预测 

分 类 号：Q938[生物学—微生物学]