α-月桂酸单甘油酯和吡咯喹啉醌二钠对蛋鸡肝脏脂质代谢的影响  

Effect ofα-monoglyceride laurate and pyrroloquinoline quinone disodium on lipid metabolism in liver of laying hens

作　　者：吴炜杭 商煜波 林继和 吴成狄 叶超群 王友明[1] 李奎 WU Wei-hang;SHANG Yu-bo;LIN Ji-he;WU Cheng-di;YE Chao-qun;WANG You-ming;LI Kui

机构地区：[1]浙江大学动物科学学院,浙江杭州310058 [2]泰顺县农业农村局,浙江温州325500 [3]浙江省畜牧技术推广与种畜禽监测总站,浙江杭州310000

出　　处：《饲料研究》2023年第15期29-35,共7页Feed Research

基　　金：2020年度科技对口支援及东西部扶贫协作项目“阿克苏蛋鸡安全高效生产关键技术研究和示范(项目编号:2020C26004)”;2021浙江省重大协同推广项目“富硒仿土鸡蛋技术研究和示范”。

摘　　要：试验旨在探索α-月桂酸单甘油酯(α-GML)和吡咯喹啉醌二钠(PQQ·Na_(2))以及两者复配对产蛋后期海兰褐蛋鸡肝脏脂质代谢的影响。选取384只体重相近健康的52周龄海兰褐蛋鸡,随机分为4组,每组4个重复,每个重复24只鸡。Con组蛋鸡喂基础饲粮;GML组、PQQ组、GML+PQQ组分别基础饲粮中添加800 mg/kg的α-GML、0.16 mg/kg的PQQ·Na_(2)以及800 mg/kg的α-GML+0.16 mg/kg的PQQ·Na_(2)。预试期2 w,正式试验期8 w。结果显示:与Con组相比,各试验组蛋鸡血清谷草转氨酶(AST)、谷丙转氨酶(ALT)活性、总胆固醇(TC)、甘油三酯(TG)含量,肝脏TC、TG含量以及肝脂率、腹脂率显著或极显著降低(P<0.05或P<0.01);GML组蛋鸡肝脏中脂肪甘油三酯脂肪酶(ATGL)、腺苷酸活化蛋白激酶α1(AMPKα1)的mRNA表达量显著上升(P<0.05),乙酰辅酶A羧化酶(ACC)、脂肪酸合酶(FASN)、硬脂酰辅酶A去饱和酶-1(SCD-1)和固醇调节元件结合蛋白-1c(SREBP-1c)的表达量极显著或显著下降(P<0.01或P<0.05);PQQ组蛋鸡肝脏中FASN、SCD-1、肉毒碱棕榈酰转移酶-1(CPT-1)的表达量极显著或显著下降(P<0.01或P<0.05);GML+PQQ组蛋鸡肝脏中ATGL的表达量显著上升(P<0.05),ACC、FASN、SCD-1、SREBP-1c和PPARγ的表达水平显著或极显著下降(P<0.05或P<0.01)。研究表明,饲粮中添加α-GML和PQQ·Na_(2)以及两者复配均能够调节蛋鸡肝脏脂质代谢,减少肝脏脂质沉积,且两种添加剂复配使用在降低蛋鸡肝脏脂质沉积方面的效果更显著。The aim of the study was to investigate the effect ofα-GML and PQQ·Na_(2) and their combination on liver lipid metabolism in late laying Hyline brown laying hens.A total of 384 fifty-two-week-old Hyline brown laying hens with similar body weight were randomly divided into four groups with four replicates per group and 24 hens per replicate.Hens in Con group were fed with basal diet.The GML group,PQQ group and GML+PQQ group were supplemented with 800 mg/kgα-GML,0.16 mg/kg PQQ·Na_(2),800 mg/kgα-GML+0.16 mg/kg PQQ·Na_(2),respectively.The pre-test lasted for 2 w,and the formal test lasted for 8 w.The results showed that compared with control group,serum AST and ALT activity,TC and TG content,liver TC and TG content,liver fat percentage and abdominal fat percentage of laying hens in experimental groups were significantly or extremely decreased(P<0.05 or P<0.01).The mRNA expression levels ofATGL and AMPKα1 in liver of hens in GML group were significantly increased(P<0.05),while the expression levels ofACC,FASN,SCD-1 and SREBP-1c were extremely or significantly decreased(P<0.01 or P<0.05).The expression levels of FASN,SCD-1 and CPT-1 in liver of hens in PQQ group were extremely or significantly decreased(P<0.01 or P<0.05).The expression level of ATGL in liver of hens in GML+PQQ group was significantly increased(P<0.05),while the expression levels of ACC,FASN,SCD-1,SREBP-1c and PPARγwere significantly or extremely decreased(P<0.05 or P<0.01).The study indicates that dietaryα-GML and PQQ·Na_(2) and their combination can regulate liver lipid metabolism and reduce liver lipid deposition of laying hens,and the combination of the two additives has a more significant effect on reducing liver lipid deposition of laying hens.

关 键 词：蛋鸡 肝脏脂质代谢 α-月桂酸单甘油酯 吡咯喹啉醌二钠 

分 类 号：S831[农业科学—畜牧学]