高效液相色谱法测定饲料中5种磺胺类药物  

Determination Method of Five Sulfonamides in Feed by High Performance Liquid Chromatography

作　　者：陈海燕[1] 张慧宁 马春芳 陈娟[1] 杨俊华[1] 谢荣国[1] 杨奇 CHEN Haiyan;ZHANG Huining;MA Chunfang;CHEN Juan;YANG Junhua;XIE Rongguo;YANG Qi(Ningxia Veterinary Drugs and Fodder Inspection Institute,Yinchuan 750001,China)

机构地区：[1]宁夏回族自治区兽药饲料监察所,银川750001

出　　处：《饲料博览》2023年第4期51-58,63,共9页Feed Review

基　　金：宁夏自然科学基金项目“饲料中非法添加兽药多组分联检方法研究及应用”(2022AAC03721)。

摘　　要：为了高效测定饲料中磺胺类药物,采用高效液相色谱法对饲料中5种磺胺类药物进行测定,将GB/T 19542—2007方法中称样容器100 mL锥形瓶改为50 mL离心管,提取液乙腈体积50 mL改为25 mL,试验对改进后的方法进行方法学验证,该方法范围为0.1~5.0μg·mL^(-1),5个浓度的线性R2>0.9999,4个不同浓度的阳性添加回收率为85.8%~113.0%,变异系数(RSD)为0.2%~7.6%,RSD<15%。结果表明改进后的方法线性好、准确性高、精密度良好,符合方法学要求。方法改进后便于旋涡、振摇、离心,提取液减量使用减少了有机试剂污染。建立了更加合理、利于操作、快捷的磺胺类药物的检测方法,可用于饲料中非法添加磺胺类药物确证检测。In order to determine sulfonamides in feed efficiently,five kinds of sulfonamides in feed were determined by high performance liquid chromatography(HPLC).The sample container 100 mL conical flask was changed into 50 mL centrifuge tube by GB/T 19542—2007 method,and the acetonitrile volume of extract was changed from 50 mL to 25 mL.The methodology of the improved method was verified.The range of the method was from 0.1 to 5.0μg·mL^(-1),R2>0.9999 for five concentrations,positive addition recoveries for four different concentrations ranged from 85.8%to 113.0%,coefficient of variation(RSD)ranged from 0.2%to 7.6%,RSD<15%.The results showed that the method had good linearity,high accuracy,good precision,and met the requirements of methodology.The improved method was convenient for whirling,shaking and centrifugation,and the organic reagent pollution was reduced by reducing the amount of the extract.A more reasonable,convenient and efficient method for the detection of sulfonamides was established,which could be used for the confirmation and detection of illegal addition of sulfonamides in feed.

关 键 词：饲料 磺胺类药物 高效液相色谱法 方法验证 

分 类 号：S816.17[农业科学—饲料科学]