六妹羊肚菌多孢分离和组织分离菌株栽培出菇试验  

Fruiting Experiment of Morchella sextelata Multispore Isolated and Tissue Isolated Strains

作　　者：王青 李闯 汪国莲[1,2] WANG Qing;LI Chuang;WANG Guo-lian(Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu,Huai’an 223001,China;Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection,Huaiyin Normal University,Huai’an 223001,China)

机构地区：[1]江苏徐淮地区淮阴农业科学研究所,江苏淮安223001 [2]淮阴师范学院区域现代农业与环境保护协同创新中心,江苏淮安223001

出　　处：《中国食用菌》2023年第4期80-84,共5页Edible Fungi of China

基　　金：江苏徐淮地区淮阴农业科学研究所科研发展基金项目(HNY202004)。

摘　　要：对3个商业化栽培羊肚菌菌株进行了鉴定,通过多孢分离及组织分离2种方式分离菌株,并利用冬闲蔬菜大棚进行栽培试验。结果表明,3个羊肚菌菌株均鉴定为六妹羊肚菌。2种分离方式下,六妹羊肚菌HY8后代分离菌株产量均高于菌株HA1和HY5。菌株HY8多孢分离后代菌株产量明显低于组织分离菌株,而菌株HA1和HY5多孢分离菌株产量略高于组织分离菌株。说明六妹羊肚菌不同菌株的大田表现差异较大,不同分离方式的后代菌株产量也存在一定差异。研究结果为羊肚菌生产中选择表现优良的菌株提供参考。Three commercially cultivated Morchella spp.strains were identitified.The strains were isolated by multispore isolation and tissue isolation methods,and cultivated by using vegetable greenhouse unoccupied in winter.Results indicated that three strains were identified as the Morchella sextelata.Under the two isolation methods,the yield of descendant strains isolated from strain HY8 were both higher than that of strain HA1 and HY5.The M.sextelata HY8 yield of multispore isolated descendant strains were obviously lower than the tissue isolated descendant ones,however the opposite situations were occurred both in HA1 and HY5 strains.Above results demonstrated that there is huge differences between field fruiting performances in different M.sextelata strains,as well as in the different isolated methods.The research result could provide reference to choose well performed strains in morels production.

关 键 词：六妹羊肚菌 多孢分离 组织分离 交配型基因 大棚栽培 

分 类 号：S646.9[农业科学—蔬菜学]