玻璃化冷冻保存对卵母细胞表观遗传修饰及转录组的影响  

Effects of vitrification on epigenetic modification and transcriptome of oocytes

作　　者：张菊 刘玉兵 千日成 Zhang Ju;Liu Yubing;Chian Ri-cheng(The First Clinical College,Medical College of Soochow University,Suzhou 215006,China;Center for Reproductive Medicine,the Tenth People's Hospital of Tongji University,Shanghai 200072,China)

机构地区：[1]苏州大学医学院第一临床学院,苏州215006 [2]同济大学附属第十人民医院生殖医学中心,上海200072

出　　处：《中华生殖与避孕杂志》2023年第8期860-865,共6页Chinese Journal of Reproduction and Contraception

基　　金：国家重点研发计划(2017YFC1002003);国家自然科学基金(31601197)。

摘　　要：卵母细胞玻璃化冷冻已经成为女性生育力保存的一线方案,在辅助生殖中得到广泛运用。卵母细胞是胚胎发育的基础,在胚胎发育早期表观遗传重编程和母源-合子转换(maternal-to-zygotic transition,MZT)中发挥重要作用,是个体发育的关键。卵母细胞表观遗传修饰和转录组对玻璃化冷冻诱导的应激非常敏感,作为一种外界强刺激,玻璃化冷冻保存可能对卵母细胞的表观遗传修饰和转录组产生不良影响。本文主要对玻璃化冷冻程序对卵母细胞表观遗传修饰和转录组的影响进行综述,为玻璃化冷冻技术的优化提高提供理论基础。Oocyte vitrification has developed as a first-line solution for female fertility preservation,which is widely used in assisted reproductive technology.As the basis of embryonic development,oocytes play an important role in early embryonic development,including epigenetic reprogramming and maternal-to-zygotic transition(MZT).They deserve the key to individual development.Oocyte epigenetic modification and transcriptome are very sensitive to stress induced by vitrification.Vitrification cryopreservation may have adverse effects on the epigenetic modification and transcriptome of oocytes as a strong external stimulus.Through the paper,we mainly reviewed the effects of vitrification on epigenetic modification and transcriptome of oocytes,hoping to provide a theoretical basis for the optimization and improvement of vitrification technology.

关 键 词：卵母细胞 玻璃化冷冻 表观遗传学 转录组 

分 类 号：R714.8[医药卫生—妇产科学]