量子点探针在病原微生物检测中的应用进展  

Progress in the application of quantum dot probes in the detection of pathogenic microorganisms

作　　者：杨宁 孟子欣蓉 赵康辰[2] 崔仑标[2] 葛以跃 Yang Ning;Meng Zixinrong;Zhao Kangchen;Cui Lunbiao;Ge Yiyue(School of Public Health Nanjing Medical University,Nanjing 210029,China;NHC Key Laboratory of Enteric Pathogen Microbiology,Jiangsu Provincial Center for Disease Control and Prevenrion,Nanjing 210029,China)

机构地区：[1]南京医科大学公共卫生学院,南京210029 [2]江苏省疾病预防控制中心、国家卫生健康委员会肠道病原微生物重点实验室,南京210029

出　　处：《中华实验和临床病毒学杂志》2023年第4期461-468,共8页Chinese Journal of Experimental and Clinical Virology

基　　金：江苏省卫健委重点科研项目(ZD2021060);江苏省六大人才高峰项目(WSN-024)。

摘　　要：随着对纳米技术的不断研究,应用于各种生物分子检测的纳米传感器层出不穷。作为主要的纳米传感器之一,量子点具有独特的光学性质和粒子特性,将其制备为量子点探针用于病原微生物检测已经获得了广泛的应用。量子点探针作为新兴的荧光标志物,拥有其他荧光探针无法比拟的优势,如荧光信号强、光稳定性好、荧光寿命长、斯托克斯位移大等,可以实现对靶标简单、快速、灵敏、特异的检测。本文概述了量子点的基本性质和量子点探针作为检测传感器的应用基础,重点介绍了量子点探针的种类、制备方法、以及近几年来在病原微生物检测中的应用,最后总结了量子点探针作为检测传感器的应用注意事项,并展望了量子点探针在病原微生物检测领域的应用前景。With the continuous research on nanotechnology,nanosensors for the detection of various biomolecular have emerged one after another.As one of the main nanosensors,quantum dots have unique optical and particle properties,and quantum dot probes for pathogen detection have been widely used.The emerging fluorescent biomarkers,quantum dot probes,have unparalleled advantages over other fluorescent probes,such as strong fluorescence signal,good photostability,long fluorescence lifetime,and large Stokes shift.They can detect the target simply,quickly,sensitively and specifically.This article provides an overview of the basic properties of quantum dots and the application foundation of quantum dot probes as detection sensors.We focus on the types and preparation method of quantum dot probes,as well as their applications in pathogen detection in recent years.Finally,the application precautions of quantum dot probes as detection sensors were summarized,and the application prospects of quantum dot probes in the field of pathogen detection were also discussed.

关 键 词：量子点 探针 检测 传感器 病原微生物 

分 类 号：R446.5[医药卫生—诊断学]