花生KNOX基因家族的全基因组鉴定及组织表达分析  

Genome-Wide Identification and Tissue Expression Analysis of KNOX Gene Family in Arachis hypogaea

作　　者：宋晓峰 鲁成凯[1] 董晓娜 高晓东[1] 孙熙文 付春[1] Song Xiaofeng;Lu Chengkai;Dong Xiaona;Gao Xiaodong;Sun Xiwen;Fu Chun(Weifang Academy of Agricultural Sciences,Weifang 261071,China)

机构地区：[1]潍坊市农业科学院,山东潍坊261071

出　　处：《山东农业科学》2023年第8期1-10,共10页Shandong Agricultural Sciences

基　　金：潍坊市科技发展计划项目(2022ZJ1087);潍坊市鸢都学者青年专家项目;财政部和农业农村部:国家现代农业产业技术体系项目(CARS-13);潍坊市现代种业创新发展项目(2021)。

摘　　要：为研究花生KNOX基因家族在生长发育以及非生物胁迫响应中的功能,本研究采用生物信息学手段在栽培种花生基因组水平上对KNOX基因家族成员进行鉴定,并对其理化性质、基因结构、蛋白质保守结构域、系统进化、启动子顺式作用元件以及组织与逆境下的表达模式进行分析。结果显示:花生基因组中共有26个KNOX基因家族成员,分布在17条染色体上,绝大多数编码酸性蛋白,均为亲水性蛋白,并全部定位于细胞核。系统发育分析可将花生KNOX家族分为ClassⅠ和ClassⅡ两个亚家族,并进一步分为4个亚组。启动子分析发现一些与生长发育、激素和胁迫响应相关的顺式作用调控元件。全生育期组织表达分析结果显示,ClassⅠ类基因表达组织特异性明显,主要在花、营养茎尖、生殖茎尖、果针等组织中高表达;ClassⅡ类基因时空表达更广泛。在7种非生物胁迫处理下,部分基因表达量变化较大,其中5个基因响应特定胁迫,RT-qPCR验证了2个特异性响应PEG胁迫的基因。本研究为进一步探究花生KNOX基因在生长发育、逆境响应中的功能奠定了基础。In order to investigate the role of KNOX gene family in peanut growth,development and abiot-ic stress response,the bioinformatics method was used to identify the KNOX gene family members at the ge-nomic level of cultivated peanuts,and their physicochemical properties,gene structures,protein conserved domains,systematic evolution,cis-acting elements of promoters,gene organizations and stress expression pat-terns were analyzed.The results showed that there were 26 KNOX gene family members in peanut genome,which distributed on 17 chromosomes most of them encoded acidic proteins,and all they were hydrophilic pro-teins and located in the nucleus.According to phylogenetic analysis,the peanut KNOX family could be divid-ed into two subfamilies,Class I and Class II,and further divided into four subgroups.Promoter analysis found some cis-acting regulatory elements related to growth and development,hormone and stress response.The re-sults of tissue expression analysis during the whole growth period showed that the expression of Class I genes were tissue-specific,and mainly highly expressed in flowers,vegetative shoot tips from main stem,reproduc-tive shoot tips from first latera,fruit needle and other tissues.Nevertheless,the temporal and spatial expres-sion of Class II genes were more extensive.Under 7 abiotic stress treatments,the expression levels of some genes changed greatly;among which,five genes responded to specific stress,and two genes specifically re-sponded to PEG stress which were verified by RT-qPCR test.This study laid a foundation for further exploring the function of KNOX genes in peanut growth,development and stress response.

关 键 词：花生 KNOX基因家族 全基因组鉴定 生物信息学分析 表达分析 

分 类 号：S565.2[农业科学—作物学] Q781[生物学—分子生物学]