小麦超氧化物歧化酶基因家族鉴定及盐胁迫下响应锌钾的表达分析  

Identification of Wheat Superoxide Dismutase Family Genes and Their Expression Analysis in Response to Zinc and Potassium under Salt Stress

作　　者：杨林林[1] 韩敏琦[1] 高嘉 杨胜敏[1] Yang Linlin;Han Minqi;Gao Jia;Yang Shengmin(Beijing Vocational College of Agriculture,Beijing 102442,China;Beijing Qinghe Water Conservancy Construction Group Co.,Ltd.,Beijing 100192,China)

机构地区：[1]北京农业职业学院,北京102442 [2]北京清河水利建设集团有限公司,北京100192

出　　处：《山东农业科学》2023年第8期11-20,共10页Shandong Agricultural Sciences

基　　金：北京市农业科技项目(20170126);北京市服务新型生产经营主体科技能力提升项目(20170203)。

摘　　要：超氧化物歧化酶(SOD)是抗氧化系统的关键酶,在保护植物免受各种生物和非生物胁迫方面发挥着至关重要的作用。然而关于小麦(Triticum aestivum L.)SOD基因家族对盐胁迫的表达模式及响应锌(Zn)和钾(K)的功能知之甚少。本研究以‘西农979’为试验材料,基于全基因组分析,对小麦SOD家族成员进行鉴定和生物信息学分析,并分析相关基因在盐胁迫下响应Zn、K的表达情况。结果表明,在小麦中共鉴定出10个SOD基因(TaSDs),包括4个Cu/Zn-SOD基因(TaCSD1~TaCSD4)、4个Fe-SOD基因(TaFSD1~TaFSD4)和2个Mn-SOD基因(TaMSD1、TaMSD2)。系统发育分析表明,TaSDs可分为Cu/Zn-SODs、Fe/Mn-SODs两大类,共7个亚组。基因结构、序列基序及启动子分析表明,所有TaSDs都具有1~7个内含子和2~7个外显子,不同TaSDs具有不同的氨基酸序列组成和高度保守的活性位点残基,且每个TaSDs的启动子中存在许多与应激反应和植物激素相关的顺式作用元件。转录组表达谱分析表明,不同TaSDs对盐分表现出不同的表达模式,TaCSD3可能是响应盐胁迫的关键基因;经RT-qPCR分析明确,TaCSD3是响应盐胁迫的靶向SOD基因,同时受Zn、K协同正向调控。本研究结果可为后续小麦耐盐育种改良提供理论依据。Superoxide dismutase(SOD)is a key enzyme in the antioxidant system that plays a vital role in protecting plants from various biotic and abiotic stresses.However,little is known about the expression pat-tern of SOD gene family in wheat(Triticum aestivum L.)under salt stress and its function in response to zinc(Zn)and potassium(K).In this study,with Xinong 979 as experimental material,the members of wheat SOD family were identified,and their bioinformatics analysis was carried out based on the whole genome anal-ysis.The expression of related genes in response to Zn and K under salt stress were analyzed,too.The results showed that a total of 10 SOD genes(TaSDs)were identified in wheat,including 4 Cu/Zn-SOD genes(TaCSD1-TaCSD4),4 Fe-SOD genes(TaFSD1-TaFSD4)and 2 Mn-SOD genes(TaMSD1 and TaMSD2).Phylogenetic analysis showed that TaSDs could be divided into Cu/Zn-SODs and Fe/Mn-SODs with 7 sub-groups.The analyses of gene structure,sequence motifs and promoters showed that all TaSDs had 1 to 7 introns and 2 to 7 exons,and different TaSDs had different amino acid sequence composition and highly con-served active site residues;there were many cis-acting elements related to stress response and plant hormones in the promoter of each TaSD.Transcriptome analysis profile analysis showed that different TaSDs showed dif-ferent expression patterns to salt stress,and TaCSD3 might be a key gene in response to salt stress.According to RT-qPCR analysis,TaCSD3 was a targeted SOD gene in response to salt stress and was positively regulated by Zn and K synergistically.The results of this study could provide a theoretical basis for the subsequent im-provement of wheat salt tolerance breeding.

关 键 词：小麦 超氧化物歧化酶 基因组 盐胁迫 锌-钾相互作用 

分 类 号：Q781[生物学—分子生物学]