基于PCR-HRM的水稻氮高效基因NRT1.1B功能标记开发及应用  

Development and Application of Functional Marker of Rice Nitrogen-Efficient Gene NRT1.1B Based on PCR-HRM

作　　者：房文文 王海凤 郭涛[1] 薛芳 姜艳芳[1] 张焕霞 张士永[1] Fang Wenwen;Wang Haifeng;Guo Tao;Xue Fang;Jiang Yanfang;Zhang Huanxia;Zhang Shiyong(Shandong Institute of Wetland Agriculture and Ecology,Shandong Academy of Agricultural Sciences/Shandong Rice Engineering Technology Research Center,Jinan 250100,China)

机构地区：[1]山东省农业科学院湿地农业与生态研究所/山东省水稻工程技术研究中心,山东济南250100

出　　处：《山东农业科学》2023年第8期21-26,共6页Shandong Agricultural Sciences

基　　金：国家水稻产业技术体系济宁试验站项目(CARS-01-080);济宁市重点研发计划项目(2021NYNS016,2020NYNS004);山东省农业科学院农业科技创新工程项目(CXGC2023A04);山东省重点研发计划(乡村振兴科技创新提振行动计划)项目(2022TZXD0040);山东省农业良种工程项目(2019LZGC003)。

摘　　要：水稻对氮肥的高效与合理利用是发展绿色农业的重要保障。培育含有氮高效基因的水稻品种,是提高氮肥利用率最经济有效的途径。本研究开发了一个用于HRM(高分辨率熔解曲线)高通量检测硝酸盐转运蛋白基因NRT1.1B的分子功能标记。利用此标记对78份山东地方水稻品种进行NRT1.1B基因分型,发现有13个水稻品种为含有NRT1.1B的高效单倍型,表明NRT1.1B在山东省水稻品种中分布较少。利用测序分析验证,测序结果与本标记分型结果一致。可见,本研究开发的功能标记能够快速准确鉴定出NRT1.1B基因型,可为筛选和培育氮高效水稻新品种提供有力的技术支撑。The efficient and reasonable utilization of nitrogen fertilizer in rice is an important guarantee for the development of green agriculture.The most economical and effective way to improve the utilization rate of nitrogen fertilizer is breeding rice varieties with nitrogen-efficient genes.In this study,a molecular function-al marker was developed for the high-throughput detection of nitrate transporter gene NTR1.1B with high reso-lution melting curve(HRM).The NRT1.1B genotyping was performed for 78 Shandong local rice varieties,and 13 varieties contained NRT1.1B high-efficient haplotypes,which indicated that NRT1.1B was less distrib-uted in Shandong rice varieties.The sequencing results were consistent with the genotyping results using this marker.These results indicated that the functional marker developed in this study could quickly and accurately identify the genotype of NRT1.1B,which could provide strong technical support for screening and breeding new nitrogen-efficient rice varieties.

关 键 词：水稻 高分辨率熔解曲线(HRM) 氮高效基因NRT1.1B 分子功能标记 

分 类 号：S511[农业科学—作物学] Q781[生物学—分子生物学]