三种微生物杀虫剂对三种小花蝽的安全性评价  

Safety Evaluation of Three Microbial Insecticides to Three Orius sauteri Species

作　　者：李郭雨 王瑞娟 陈浩[2] 刘艳[2] 吕兵[2] 代晓彦[2] 苏龙 赵珊 郑礼[2] 董小林[1] 翟一凡 Li Guoyu;Wang Ruijuan;Chen Hao;Liu Yan;LüBing;Dai Xiaoyan;Su Long;Zhao Shan;Zheng Li;Dong Xiaolin;Zhai Yifan(College of Agronomy,Yangtze University/Forewarning and Management of Agricultural and Forestry Pests,Hubei Engineering Technology Center,Jingzhou 434000,China;Institute of Plant Protection,Shandong Academy of Agricultural Sciences/Key Laboratory of Natural Enemies Insects,Ministry of Agriculture and Rural Affairs,Jinan 250100,China)

机构地区：[1]长江大学农学院/湖北省农林病虫害预警与调控工程技术研究中心,湖北荆州434000 [2]山东省农业科学院植物保护研究所/农业农村部天敌昆虫重点实验室,山东济南250100

出　　处：《山东农业科学》2023年第8期129-134,共6页Shandong Agricultural Sciences

基　　金：山东省农业科学院农业科技创新工程项目(CXGC2022E04);济南市农业应用技术创新项目(CX202201);上海市科技兴农推广项目(沪农科推字[2021]第2-4号);2021年度山东省乡村振兴科技创新提振行动计划项目(2021TZXD007)。

摘　　要：为了探究微生物杀虫剂对天敌昆虫小花蝽的安全性,为微生物杀虫剂与小花蝽联合使用防治害虫提供理论依据,本试验在实验室条件下采用浸虫法测定球孢白僵菌(Beauveria bassiana)、金龟子绿僵菌(Metarhizium anisopliae)和苏云金杆菌(Bacillus thuringiensis)对微小花蝽(Orius minuius)、南方小花蝽(Orius similis)、东亚小花蝽(Orius sauteri)的毒力,比较其毒性大小,并比较了田间推荐使用剂量下用药7 d对小花蝽的致死率,评价其安全性。结果表明,球孢白僵菌对东亚小花蝽毒性最小,LC_(50)为2.88×10^(8)CFU/mL;金龟子绿僵菌对微小花蝽毒性最小,LC_(50)为5.20×10^(5)CFU/mL;苏云金杆菌对微小花蝽的毒性最小,LC50为1.24×10^(5)IU/mL。按照国际生物防治组织的评价标准,球孢白僵菌对东亚小花蝽的致死率为25.13%,属于无害,但对其余两种小花蝽轻度有害;金龟子绿僵菌与苏云金杆菌对三种小花蝽均为轻度有害。This study was aimed to explore the safety of microbial insecticides against the natural enemy insect Orius,and provide a theoretical basis for the combined use of microbial insecticides and natural enemy to control pests.The immersion method was adopted under laboratory conditions to determine and compare the virulence of Beauveria bassiana,Metarhizium anisopliae and Bacillus thuringiensis to Orius minuius,Orius si-milis and Orius sauteri.The mortality rate was also compared at the field recommended dose after 7 days of ap-plication to evaluate their safety.The results showed that B.bassiana had the lowest toxicity to O.sauteri with the value of LC_(50)as 2.88×10^(8)CFU/mL,M.anisopliae had the lowest toxicity to O.minuius with the value of LC_(50)as 5.20×10^(5)CFU/mL,and B.thuringiensis had the lowest toxicity to O.minuius with the value of LC50 as 1.24×10^(5)IU/mL.The mortality rate of B.bassiana to O.sauteri was 25.13%,which belonged to harmless level,according to the assessment standard of the International Organization for Biological Control,but it was slightly harmful to the other two Orius species,M.anisopliae and B.thuringiensis were both slightly harmful to all the tested Orius.

关 键 词：微生物杀虫剂 天敌小花蝽 毒性试验 安全性评价 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]