不同LED组合光质对莴苣灰霉病防御酶活性的影响  

Effects of Different LED Light Combinations on Activity of Defense Enzymes against Botrytis cinerea of Lactuca sativa L.

作　　者：刘勇鹏 王彬[2] 杨哲 任旭妍 朱新红 马肖静[3] 孙凯乐 孙治强[3] 朴凤植[3] 张涛[3] 姚秋菊[2] Liu Yongpeng;Wang Bin∗;Yang Zhe;Ren Xuyan;Zhu Xinhong;Ma Xiaojing;Sun Kaile;Sun Zhiqiang;Piao Fengzhi;Zhang Tao;Yao Qiuju(Luohe Academy of Agricultural Sciences,Luohe 462000,China;Horticulture Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China;Henan Zhumadian Agricultural School,Zhumadian 463000,China)

机构地区：[1]漯河市农业科学院,河南漯河462000 [2]河南省农业科学院园艺研究所,河南郑州450002 [3]河南农业大学园艺学院,河南郑州450002 [4]河南省驻马店农业学校,河南驻马店463000

出　　处：《山东农业科学》2023年第8期141-147,共7页Shandong Agricultural Sciences

基　　金：国家特色蔬菜产业技术体系郑州综合试验站(CARS-24-G-15);河南省科技攻关项目(212102110406)。

摘　　要：本试验在水培条件下,以LED红蓝光(红蓝光比例4∶1)为基础和对照,研究添加紫光、黄光、绿光组成的不同LED组合光质对叶用莴苣接种灰霉病菌后的病情指数以及接菌前后POD、PPO、PAL等相关防御酶活性的影响。结果表明,不同LED组合光质处理中,以添加紫光的LED红蓝紫处理最能降低莴苣灰霉病病情指数;防御酶活性上,红蓝光基础上添加紫光和黄光处理的POD、PAL、PPO、GLU活性较高,添加绿光、黄光处理的CHT活性较高。整体上看,在LED红蓝光基础上添加一定比例的紫光或黄光,不仅可以有效降低莴苣灰霉病的病情指数,还可以提高莴苣体内的防御酶活性。该结果可为莴苣生产中通过光环境控制来抑制灰霉病发生提供理论参考。In this experiment,using the hydroponics,the effects of adding different LED light such as purple light,yellow light and green light on the disease index of Lactuca sativa L.inoculated with Botrytis cine-rea and the activities of related defense enzymes such as POD,PPO and PAL before and after inoculation were studied on the basis of LED red and blue light combination(the ratio of red to blue light as 4∶1).The results showed that among the different LED light combinations,adding purple LED light was the best to reduce the disease index of L.sativa gray mold.In terms of defense enzyme activity,the POD,PAL,PPO and GLU ac-tivities were higher by adding purple light and yellow light,and the CHT activity was higher by adding green and yellow light.To sum up,adding a certain proportion of purple or yellow light on the basis of LED red and blue light combination could not only effectively reduce the disease index of gray mold of lettuce,but also im-prove the defense enzyme activities in lettuce.These results could provide an important theoretical basis for controlling the occurrence of gray mold in lettuce production through the regulation of light environment.

关 键 词：LED 组合光质 莴苣 灰霉病 防御酶 

分 类 号：S436.31[农业科学—农业昆虫与害虫防治]