针刺“百会”穴联合黄精多糖对高脂饮食诱导的学习记忆障碍大鼠肠道菌群的影响  

Effect of Needling DU20 Combined with Polysaccharide from Polygonatum Sibiricum on Gut Microbiota of Rats with Learning and Memory Impairment Induced by High-Fat Diet

作　　者：王迪 周忠光[2] 杨圆圆 郑时嘉 韩增鑫 赵健[5] WANG Di;ZHOU Zhongguang;YANG Yuanyuan;ZHENG Shijia;HAN Zengxin;ZHAO Jian(The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150060,China;Heilongjiang University of Chinese Medicine,Harbin 150040,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Tianwenshan Agricultural Comprehensive Development Co.,Ltd.,Harbin 150323,China;The Fourth Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150010,China)

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150060 [2]黑龙江中医药大学,黑龙江哈尔滨150040 [3]北京中医药大学东直门医院,北京100700 [4]天问山农业综合开发股份有限公司,黑龙江哈尔滨150323 [5]黑龙江中医药大学附属第四医院,黑龙江哈尔滨150010

出　　处：《针灸临床杂志》2023年第8期70-77,共8页Journal of Clinical Acupuncture and Moxibustion

基　　金：黑龙江省博士后基金资助项目,编号:LBH-Z19101;黑龙江省应用技术研究与开发技术重大项目,编号:GA20C017、GA16C102;黑龙江省卫生健康委科研课题,编号:20220202041110。

摘　　要：目的:观察学习记忆障碍模型大鼠肠道菌群的变化,探究学习记忆障碍与肠道菌群的关系,揭示针刺“百会”穴联合黄精多糖治疗改善学习记忆能力的可能机制。方法:将SD大鼠按实验要求分为空白组、模型组和针药组(n=6),利用高脂饮食喂养8周诱导学习记忆障碍模型,治疗开始后每日对应给予生理盐水、针刺“百会”联合黄精多糖,3周后Morris水迷宫进行行为学检测;HE染色观察脑组织病理形态学改变;16S rDNA测序检测大鼠肠道菌群的改变。结果:模型组大鼠的逃避潜伏期时间明显延长、穿越平台次数明显减少(P<0.05),针药治疗后大鼠的逃避潜伏期时间明显缩短、穿越平台次数明显增多,差异具有统计学意义(P<0.05);在属水平上,与空白组相比,模型组大鼠肠道菌群中Bacteroides、Coprococcus_3、UBA1819、Sutterella、Coprococcus_2、Holdemania、Gemella、Family_XIII_AD3011_group、Corynebacterium_1、Family_XIII_UCG_001、Eubacterium_hallii_group、Candidatus_Stoquefichus、Erysipelatoclostridium、Lachnospira、Lachnospiraceae_ND3007_group、Ruminococcaceae_UCG_008及Staphylococcus相对丰度显著升高,差异具有统计学意义(P<0.05),Prevotellaceae_UCG_001、Prevotellaceae_Ga6A1_group、Pygmaiobacter、Alloprevotella及Butyrivibrio相对丰度显著降低,差异具有统计学意义(P<0.05);针药治疗后,模型组大鼠肠道菌群中相对丰度显著升高的菌属相对丰度均下降,差异具有统计学意义(P<0.05),Lachnospiraceae_NK4A136_group、Lachnospiraceae_UCG_006、Ruminiclostridium_5、Anaerostipes、Ruminococcaceae_UCG_013、Candidatus_Saccharimonas及Bacteroides_pectinophilus_group相对丰度显著升高,差异具有统计学意义(P<0.05)。结论:高脂饮食可能通过破坏肠道菌群稳态,导致肠道菌群紊乱,影响与认知相关的代谢产物及炎性因子产生,从而引起学习记忆障碍。针刺“百会”穴联合黄精多糖治疗能够改善学习记忆能力,治疗机制与�Objective:To observe the changes of gut microbiota of rats with learning and memory impairment,to explore the relationship between learning and memory impairment and gut microbiota,thus to reveal the possible mechanism of needling DU20 combined with polysaccharide from Polygonatum Sibiricum in improving learning and memory ability.Methods:SD rats were divided into the blank group,the model group and the acupuncture-medication group(n=6).The learning and memory impairment model was induced by high-fat diet for 8 weeks.After modeling,the blank group and the model group were given normal saline,and the acupuncture-medication group was treated with polysaccharide from Polygonatum Sibiricum and needling DU20.After 3 weeks of intervention,Morris water maze was used for behavioral test,and HE staining was used for observing the pathological changes of brain tissues,and 16S rDNA sequencing technology was used for detecting the changes of gut microbiota.Results:The escape latency was significantly prolonged,and the times of crossing the platform were significantly reduced in the model group(P<0.05).After the acupuncture-medication intervention,the escape latency was significantly shortened,the times of crossing the platform were significantly increased(P<0.05).In terms of the genus level,compared with those in the blank group,the relative abundance of Bacteroides,Coproccus_3,UBA1819,Sutterella,Coproccus_2,Holdemania,Gemella,Family_XIII_AD3011_group,Corynebacterium_1,Family_XIII_UCG_001,Eubacterium_hallii_group,Candidatus_Stoquefichus,Erysipelatoclostridium,Lachnospira,Lachnospiraceae_ND3007_group,Ruminococoaceae_UCG_008 and Staphylococcus were increased significantly(P<0.05);whereas the relative abundance of Prevotellaceae_UCG_001,Prevotellaceae_Ga6A1_group,Pygmaiobacter,Alloprevotella and Butyrivibrio were decreased significantly(P<0.05).After the acupuncture-medication intervention,the increasing relative abundance of microbiota of rats were decreased(P<0.05),and the relative abundance of Lachnospiraceae_NK4A136_group,L

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分 类 号：R246.6[医药卫生—针灸推拿学]