深圳市3种不同林分丘陵林地土壤细菌群落特征  

Characteristics of soil bacterial communities in hilly woodlands of three different forest stands in Shenzhen

作　　者：董志强 史正军[2,3] 谢惠春 曾伟 冯世秀[2] 邓丽 DONG Zhi-qiang;SHI Zheng-jun;XIE Hui-chun;ZENG Wei;FENG Shi-xiu;DENG Li(College of Life Sciences,Qinghai Normal University,Xining 810008,China;Key Laboratory of Southern Subtropical Plant Diversity Fairylake Botanical Garden,Shenzhen&Chinese Academy of Sciences,Shenzhen,Guangdong 518004,China;China Shenzhen Urban Forest Ecosystem Research Station,State Administration of Forest and Grassland,Shenzhen,Guangdong 518004,China)

机构地区：[1]青海师范大学生命科学学院,西宁810008 [2]深圳市中国科学院仙湖植物园/深圳市南亚热带植物多样性重点实验室,广东深圳518004 [3]广东深圳城市森林生态系统国家定位观测研究站,广东深圳518004

出　　处：《西南农业学报》2023年第7期1432-1437,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2021YFE0193200);深圳市城管和综合执法局科研项目(202018);仙湖植物园科研基金项目(FLSF-2021-03)。

摘　　要：【目的】揭示不同恢复模式下林地土壤细菌群落环境的作用机理。【方法】利用高通量测序技术,通过南亚热带丘陵土沉香林(Aquilaria sinensis)、马占相思林(Acacia mangium)和天然次生林地土壤细菌群落组成及多样性,分析土壤理化性质与优势菌群的关系。【结果】①在不同林分土壤细菌分类研究中,土沉香林、马占相思林、次生林土壤细菌分别有44、32和35个门。3种林分土壤细菌组成较为相似,优势细菌菌群均为变形菌门(Proteobacteria)和醋酸菌门(Acidobacteria),相对丰度均>1%的群落均为放线菌门(Actinobacteria)、疣微菌门(Verrucomicrobia)和浮霉菌门(Planctomycetes)。②在不同林分土壤细菌α-多样性分析中,次生林土壤Chao1指数最高,表示该土壤中细菌群落数量最高,土沉香林土壤Shannon指数最高,表示该土壤中细菌多样性高。③在不同林分土壤细菌β-多样性分析中,土沉香林和马占相思林土壤细菌群落结构具有差异性,而次生林与前二者土壤细菌结构相似。④在不同林分土壤RDA冗余分析中,变形菌群和浮霉菌群与速效钾、有效磷及全磷呈显著正相关。酸杆菌门与土壤pH、有效磷和速效钾呈极显著负相关。【结论】研究结果揭示了深圳市3种不同林分土壤细菌群落组成结构差异性以及多样性,为进一步探究人工恢复模式是否优于天然林奠定理论基础。【Objective】The present paper aimed to reveal the mechanism of action of soil bacterial community environment in woodland under different restoration patterns.【Method】Using high-throughput sequencing technology,we analyzed the relationship between soil physicochemical properties and dominant bacterial communities through the composition and diversity of soil bacterial communities in southern Aquilaria sinensis,Acacia mangium forests and natural secondary woodlands.【Result】(i)In the taxonomic study of soil bacteria in different forest stands,there were 44,32 and 35 phyla of soil bacteria in the A.sinensis,A.mangium and secondary forest,respectively.The composition of soil bacteria in the three forest stands was relatively similar,and the dominant bacterial groups were Proteobacteria and Acidobacteria,and the communities with relative abundance 1%were all Actinobacteria,Verrucomicrobia and Planctomycetes.(ii)In theα-diversity analysis of soil bacteria in different forest stands,the highest Chao1 index of secondary forest soil indicated the highest bacterial community in the soil,and the highest Shannon index of soil sedum forest soil indicated high bacterial diversity in this soil.(iii)In theβ-diversity analysis of soil bacteria in different forest stands,the soil bacterial community structure of soil sedum forest and A.mangium forest was differential,while the secondary forest had similar soil bacterial structure to the former two.(iv)In the RDA redundancy analysis of soils in different stands,Phytophthora variegata and Phytophthora floxa were significantly and positively correlated with fast-acting potassium,effective phosphorus and total phosphorus.Acidobacteria were negatively and significantly correlated with soil pH,effective phosphorus and fast-acting potassium.【Conclusion】The results reveal the structural variability and diversity of soil bacterial community composition in three different forest stands in Shenzhen,and provide a theoretical basis for further exploring whether the artificial

关 键 词：南亚热带 恢复模式 土壤 细菌 群落结构 高通量测序 

分 类 号：S714.3[农业科学—林学]