3D bioprinting of complex biological structures with tunable elastic modulus and porosity using freeform reversible embedding of suspended hydrogels  

作　　者：Zhuang Chen Chuanzhen Huang Hanlian Liu Xu Han Zhichao Wang Shuying Li Jun Huang Zhen Wang 

机构地区：[1]Centre for Advanced Jet Engineering Technology(CaJET),Key Laboratory of High-Efficiency and Clean Mechanical Manufacture(Ministry of Education),National Experimental Teaching Demonstration Center for Mechanical Engineering(Shandong University),School of Mechanical Engineering,Shandong University,Jinan 250061,China [2]School of Mechanical Engineering,Yanshan University,Qinhuangdao 066004,China

出　　处：《Bio-Design and Manufacturing》2023年第5期550-562,共13页生物设计与制造（英文）

基　　金：supported by the National Natural Science Foundation of China(Nos.52275464 and 52075300);the Scientific Research Project for National High-Level Innovative Talents ofHebei Province Full-Time Introduction(No.2021HBQZYCXY004).

摘　　要：Three-dimensional(3D)bioprinting has been used widely for the construction of hard tissues such as bone and cartilage.However,constructing soft tissues with complex structures remains a challenge.In this study,complex structures characterized by both tunable elastic modulus and porosity were printed using freeform reversible embedding of suspended hydrogels(FRESHs)printing methods.A mixture of alginate and gelatin was used as the main functional component of the bioink.Rheological analysis showed that this bioink possesses shear thinning and shear recovery properties,supporting both cryogenic and FRESH printing methods.Potential printing capabilities and limitations of cryogenic and FRESH printing were then analyzed by printability tests.A series of complex structures were printed by FRESH printing methods which could not be realized using conventional approaches.Mechanical tests and scanning electron microscopy analysis showed that the printed structure is of excellent flexibility and could be applied in various conditions by adjusting its mechanical modulus and porosity.L929 fibroblast cells maintained cell viability in cell-laden-printed structures,and the addition of collagen further improved the hydrogels’biocompatibility.Overall,all results provided useful insight into the building of human soft tissue organ blocks.

关 键 词：Bioink Freeform reversible embedding of suspended hydrogels(FRESHs)printing 3D extrusion cell-laden printing Tissue engineering Tunable elastic modulus and porosity 

分 类 号：R318[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]