Advanced strategies in the application of gelatin-based bioink for extrusion bioprinting  

作　　者：Jirong Yang Huimin He Duo Li Qian Zhang Lizhi Xu Changshun Ruan 

机构地区：[1]Research Center for Human Tissue and Organs Degeneration,Institute of Biomedicine and Biotechnology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [2]Department of Mechanical Engineering,The University of Hong Kong,Hong Kong SAR,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Bio-Design and Manufacturing》2023年第5期586-608,共23页生物设计与制造（英文）

基　　金：support for this work from the National Key R&D Program of China(No.2018YFA0703100);the National Natural Sci-ence Foundation of China(Nos.32122046,82072082,and 32000959);the Youth Innovation Promotion Association of CAS(No.2019350);the Guangdong Natural Science Foundation(No.2019A1515111197);the Shenzhen Fundamental Research Foun-dation(Nos.JCYJ20190812162809131,JCYJ20200109114006014,JCYJ20210324113001005,and JCYJ20210324115814040).

摘　　要：The significance of bioink suitability for the extrusion bioprinting of tissue-like constructs cannot be overemphasized.Gelatin,derived from the hydrolysis of collagen,not only can mimic the extracellular matrix to immensely support cell function,but also is suitable for extrusion under certain conditions.Thus,gelatin has been recognized as a promising bioink for extrusion bioprinting.However,the development of a gelatin-based bioink with satisfactory printability and bioactivity to fabricate complex tissue-like constructs with the desired physicochemical properties and biofunctions for a specific biomedical application is still in its infancy.Therefore,in this review,we aim to comprehensively summarize the state-of-the-art methods of gelatin-based bioink application for extrusion bioprinting.Wefirstly outline the properties and requirements of gelatin-based bioinks for extrusion bioprinting,highlighting the strategies to overcome their main limitations in terms of printability,structural stability and cell viability.Then,the challenges and prospects are further discussed regarding the development of ideal gelatin-based bioinks for extrusion bioprinting to create complex tissue-like constructs with preferable physicochemical properties and biofunctions.

关 键 词：Gelatin-based bioink Extrusion bioprinting Tissue-like construct 

分 类 号：TP391.73[自动化与计算机技术—计算机应用技术] R318[自动化与计算机技术—计算机科学与技术]