知母山楂饮对大鼠非酒精性脂肪肝的治疗作用及可能机制的研究  

Study on the therapeutic effect and mechanism of anemarrhenae-hawthorn drink on non-alcoholic fatty liver in rats

作　　者：王树根 宁美英[1] 王颖[2] 杨晓晖[3] 李梦冉 WANG Shugen;NING Meiying;WANG Ying;YANG Xiaohui;LI Mengran(Department of Pharmacy,Cangzhou Central Hospital,Cangzhou,Hebei 061000,China;Traditional Chinese Medicine Department,Cangzhou Central Hospital,Cangzhou,Hebei 061000,China;Department of Experimental Center,Cangzhou Medical College,Cangzhou,Hebei 061000,China;College of Life Sciences,Cangzhou Nor-mal University,Cangzhou,Hebei 061000,China)

机构地区：[1]沧州市中心医院药学部,河北沧州061000 [2]沧州市中心医院中医科,河北沧州061000 [3]沧州医学高等专科学校实验中心,河北沧州061000 [4]沧州师范学院河北生命科学学院,河北沧州061000

出　　处：《安徽医药》2023年第10期1927-1932,I0002,共7页Anhui Medical and Pharmaceutical Journal

基　　金：河北省中医药管理局中医药类科研计划项目(2022615)。

摘　　要：目的研究知母山楂饮对非酒精性脂肪性肝病(NAFLD)大鼠的治疗作用。方法2021年6—12月,在沧州医学高等专科学校实验中心将55只雄性SD大鼠(其中5只大鼠用于判断是否造模成功)经过适应性喂养后,按照随机数字表法分为正常组(基础饲料喂养)、模型组(高脂饲料喂养进行NAFLD模型造模)、阳性对照组(造模成功后给予普罗布考500 mg/kg)、低剂量给药组(造模成功后给予知母山楂饮2.5 g/kg)、高剂量给药组(造模成功后给予知母山楂饮5 g/kg),每组10只。造模后培养8周,对各组大鼠称体质量,同时进行胰岛素耐量试验,以及测定各组大鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、超氧化物歧化酶(SOD)、丙二醛(MDA)。之后,各组的大鼠被处死,剖取各组大鼠肝脏组织。计算肝脏指数后,经HE染色、油红O染色后观察组织病理形态学变化。并测定肝脏组织的SOD、MDA、肿瘤坏死因子α(TNF-α)和白细胞介素-6(IL-6)。对肝脏组织11β-羟基类固醇脱氢酶1(11β-HSD1)、髓样分化因子初次应答基因88(myd88)、Toll样受体4(TLR4)和核因子κB(NF-κB)mRNA表达及蛋白表达检测。结果低剂量给药组大鼠体质量(515.72±20.55)g明显低于模型组(595.26±27.88)g(P<0.01),其中低剂量给药组肝脏指数变化(2.65±0.05)%明显低于模型组(3.05±0.09)%(P<0.01);累积糖耐量低剂量给药组(14.63±1.05)mmol·L^(-1)·h^(-1)明显低于模型组(17.95±1.09)mmol·L^(-1)·h^(-1)(P<0.05),可改善胰岛素抵抗;很好改善大鼠血清中TC、TG、LDL-C、AST、ALT、MDA水平,升高HDL-C、SOD水平(P<0.05);降低肝脏组织组织中11β-HSDl、MyD88、TLR4B和NF-κB表达水平(P<0.05);并且高剂量给药组效果明显。结论知母山楂饮可以很好地改善高脂饲料引发的大鼠非酒精性肝损伤。Objective To investigate the therapeutic effect of rhizoma anemarrhenae hawthorn decoction on nonalcoholic fatty liver in rats.Methods From June to December 2021,55 male SD rats(5 of which were used to judge the success of modeling)were fed in the Experimental Center of Cangzhou Medical College.After adaptive feeding,rats were divided into normal group(fed with basal diet),model group(fed with high-fat diet for nonalcoholic fatty liver model),positive control group(treated with probucol 500 mg/kg),low dose group(treated with rhizoma anemarrhenae hawthorn decoction 2.5 g/kg)and high dose group(treated with rhizoma anemarrhenae hawthorn decoction 5 g/kg).The rats were weighed after 8 weeks of feeding.At the same time,insulin tolerance test and serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),aspartate aminotransferase(AST),glutamic pyruvic transaminase(ALT),superoxide dismutase(SOD)and malondialdehyde(MDA)were measured.Thereafter,the rats in each group were sacrificed and the liver tissues of each group were dissected.After calculating the liver index,the histopathological changes were observed after HE staining and Oil red O staining.And the SOD,MDA,TNF-αand IL-6 in the liver were measured.The mRNA and protein expression of 11b-HSDl,MyD88,TLR4B and NF-kB were detected in liver tissue.Results Compared with the model group,the positive control group and each administration group could slow down the growth of body mass of rats,and that of the low dose administration group(515.72±20.55)g was significantly lower than that of the model group(595.26±27.88)g,(P<0.01)and liver index changes.The low dose group(2.65±0.05)%was significantly lower than the model group(3.05±0.09)%(P<0.01);Insulin resistance was improved,and the cumulative glucose tolerance in low-dose administration group(14.63±1.05)mmol·L^(-1)·h^(-1) was significantly lower than that in model group(17.95±1.09)mmol·L^(-1)·h^(-1)(P<0.05).The levels of TC,TG,LDLC,AST,ALT

关 键 词：脂肪肝 知母山楂饮 大鼠 高脂血症 1β-羟基类固醇脱氢酶1(11β-HSD1) 髓样分化因子初次应答基因88(myd88) Toll样受体4(TLR4) 核因子κB(NF-κB) 

分 类 号：R285.5[医药卫生—中药学]