黄芪总皂苷对薄型子宫内膜的修复作用及机制探究  

Repair effect of total saponins of astragalus on thin endometrium and its mechanism

作　　者：刘利英 高晓 LIU Liying;GAO Xiao(Department of Gynecology,Shaanxi Nuclear Industry No.215 Hospital,Xianyang,Shaanxi 712000,China;Department of Pathology,Shaanxi Nuclear Industry No.215 Hospital,Xianyang,Shaanxi 712000,China)

机构地区：[1]陕西省核工业二一五医院妇科,陕西咸阳712000 [2]陕西省核工业二一五医院病理科,陕西咸阳712000

出　　处：《安徽医药》2023年第10期1933-1936,I0002,共5页Anhui Medical and Pharmaceutical Journal

基　　金：陕西省医学科学研究课题计划(2019JM0917)。

摘　　要：目的探讨黄芪总皂苷(total saponins of astragalus,TSA)对薄型子宫内膜的修复作用及相关机制。方法2022年1—4月,将36只雌性小鼠分为三组(n=12):假手术组(Sham组)、模型组(Model组)、治疗组(Model+TSA组)。宫腔灌注无水乙醇进行造模,治疗组小鼠腹腔注射TSA溶液(45 mg·kg^(-1)·d^(-1)),共3周。苏木精-伊红(HE)染色观察小鼠子宫内膜厚度及形态;扫描电子显微镜观察子宫内膜胞饮突;蛋白质印迹法检测各组小鼠子宫内膜容受性因子血管性血友病因子(vWF)、血管内皮生长因子(vEGF)、同源异型盒A10(HoxA10)、白血病抑制因子(LIF);观察小鼠妊娠胚胎数以评估生育力。结果与Sham组[(410.56±21.47)μm、(7.58±4.87)个、100.00±13.32、100.00±8.43、100.00±5.91、100.00±11.97、(10.98±1.24)个]相比,Model组小鼠子宫内膜厚度[(169.10±34.33)μm]、腺体数量[(1.00±0.86)个]、内膜容受性相关因子vWF(31.29±10.17)、vEGF(22.17±3.92)、HoxA10(30.26±6.37)、LIF(51.83±6.01),以及妊娠胚胎数[(2.42±1.37)个]明显降低(P<0.05);与Model组[(169.10±34.33)μm、1.00±0.86、31.29±10.17、22.17±3.92、30.26±6.37、51.83±6.01、(2.42±1.37)个]相比,Model+TSA组小鼠子宫内膜厚度[(337.89±32.18)μm)、腺体数量[(5.47±1.02)个]、vWF(69.18±5.88)、vEGF(49.40±11.37)、HoxA10(61.28±6.22)、LIF(90.29±12.77),以及妊娠胚胎数[(7.23±1.57)个]明显增加(P<0.05);Sham组小鼠子宫内膜可见成熟胞饮突表达;Model组小鼠子宫内膜未见明显成熟胞饮突,仅见少量小胞饮突表达;而Model+TSA组小鼠子宫内膜可见发育成熟的胞饮突。结论TSA可通过提高内膜容受性发挥对薄型子宫内膜的修复作用。Objective To investigate the repair effect of total saponins of astragalus(TSA)on thin endometrium and its related mechanism.Methods January to April 2022,thirty-six female mice were divided into three groups(n=12):Sham group(Sham group),Model group(Model group)and treatment group(Model+TSA group).Mice in the treatment group were intraperitoneally injected with TSA solution(45 mg·kg^(-1)·d^(-1))for 3 weeks.Endometrial thickness and morphology were observed by HE staining.The endometrial pinocytosis process was observed by scanning electron microscope.Endometrial receptivity factors von Willebrand factor(vWF),vascular endothelial growth factor(vEGF),hemeobox A10(HoxA10),and leukemia inhibitory factor(LIF)were detected by Western blotting.The number of pregnant embryos in mice was observed to evaluate their fertility.Results The endometrial thickness[(169.10±34.33)μm],number of glands[(1.00±0.86)],endometrial receptivity related factors vWF(31.29±10.17),vEGF(22.17±3.92),HoxA10(30.26±6.37),LIF(51).83±6.01),and the number of pregnant embryos[(2.42±1.37)]in the Model group were significantly decreased compared with Sham group[(410.56±21.47)μm,(7.58±4.87),100.00±13.32,100.00±8.43,100.00±5.91,100.00±11.97,(10.98±1.24)](P<0.05);Compared with Model group[(169.10±34.33)μm,1.00±0.86,31.29±10.17,22.17±3.92,30.26±6.37,51.83±6.01,(2.42±1.37)],endometrial thickness[(337.89±32.18)μm),number of glands[(5.47±1.02)],vWF(69.18±5.88),vEGF(49.40±11.37),HoxA10(61.28±6.22),LIF(90.29±12.77)and the number of pregnant embryos[(7.23±1.57)]was significantly increased in Model+TSA group(P<0.05);mature pinocytosis expression was observed in the endometrium of mice in Sham group;no obvious mature pinocytosis expression was observed in the endometrium of mice in Model group,only a few small pinocytosis expression was observed.The endometrium of Model+TSA group showed mature pinocytosis.Conclusion TSA can repair thin endometrium by increasing endometrial receptivity.

关 键 词：黄芪 黄芪总皂苷 薄型子宫内膜 胞饮突 子宫内膜容受性 小鼠 近交C57BL 

分 类 号：R285[医药卫生—中药学]