微RNA和去乙酰化在支气管哮喘中对Th细胞因子的影响  

Effects of miRNA and deacetylation on Th cytokine in bronchial asthma

作　　者：张茹 卜倩[2] 贾宏林 梁晓鹰[3] 高丽[1] 姜孝芳[1] ZHANG Ru;BU Qian;JIA Honglin;LIANG Xiaoying;GAO Li;JIANG Xiaofang(School of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi,The Xinjiang Uygur Autonomous Region,830000,China;Ophthalmolog Department,The Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University,Urumqi,Xinjiang Uygur Autonomous Region 830000,China;Rehabilitation Department,The Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University,Urumqi,Xinjiang Uygur Autonomous Region 830000,China)

机构地区：[1]新疆医科大学中医学院,新疆维吾尔自治区乌鲁木齐830000 [2]新疆医科大学附属中医医院眼科,新疆维吾尔自治区乌鲁木齐830000 [3]新疆医科大学附属中医医院康复科,新疆维吾尔自治区乌鲁木齐830000

出　　处：《安徽医药》2023年第10期1949-1955,I0003,共8页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金项目(81760182)。

摘　　要：目的探究支气管哮喘(BA)病人外周血CD4+T淋巴细胞中微RNA-126(miR-126)、微RNA-326(miR-326)和微RNA-155(miR-155)以及Th细胞相关因子的表达情况;探究去乙酰化酶抑制剂曲古抑菌素A(TSA)对支气管哮喘大鼠miR-126、miR-326和miR-155以及Th细胞相关因子的调控作用。方法收集2015年10月至2017年12月就诊于新疆医科大学第一附属医院、新疆维吾尔自治区人民医院及新疆医科大学附属中医医院的哮喘急性发作期病人(48例),采用实时荧光定量PCR技术检测BA病人miR-126、miR-326和miRNA-155以及Th细胞相关因子mRNA的表达情况。建立BA大鼠模型,分别给予大鼠低、高浓度的TSA干预,PCR技术检测各组miR-126、miR-326和miR-155以及Th细胞相关因子的表达。免疫组化法检测干扰素-γ(IFN-γ)、GATA结合蛋白3(GATA3)、维甲酸相关孤儿受体(ROR-γ)、叉头翼状螺旋转录因子3(Foxp3)蛋白表达。结果BA病人组miR-126、miR-326和miR-155 mRNA的表达高于健康人组(1.63±0.30比0.54±0.10,2.25±0.67比1.62±0.39,2.17±0.68比1.75±0.46,均P<0.05),IFN-γ和Foxp3 mRNA表达低于健康人组(0.86±0.19比1.36±0.27,1.01±0.22比1.83±0.30,P<0.05),ROR-γmRNA表达高于正常组(2.79±0.40比1.62±0.38,P<0.05);BA大鼠模型中,模型组中miR-126 mRNA的表达高于正常组(2.17±0.39比0.92±0.06,P<0.01),TSA低剂量组中miR-126 mRNA的表达低于模型组(2.17±0.39比1.24±0.20,P<0.05);TSA高剂量组中GATA3 mRNA的表达低于正常组和模型组(3.69±0.88比1.64±0.35、1.08±0.36,P<0.01);在TSA高剂量组中ROR-γmRNA的表达高于正常组和模型组(5.50±1.02比0.89±0.37、10.94±2.85,P<0.05)。结论去乙酰化酶抑制剂可下调miR-126、miR-326和miR-155的表达;适量的去乙酰化酶抑制剂可上调细胞因子IFN-γ和Foxp3的表达以及下调GATA3和ROR-γ的表达。Objective To investigate the expression of microRNA-126(miR-126),microRNA-326(miR-326),microRNA-155(miR-155)and Th cell-related factors in peripheral blood CD4+T lymphocytes from bronchial asthma(BA)patients,and to investigate the regulatory effects of the deacetylase inhibitor trichostation A(TSA)on miR-126,miR-326,miR-155 and Th cell-associated factors in bronchial asthma rats.Methods Collect 48 patients with acute asthma attacks who visited the First Affiliated Hospital of Xinjiang Medical University,the People's Hospital of Xinjiang Uygur Autonomous Region,and the Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University from October 2015 to December 2017.Real-time fluorescence quantitative PCR was used to detect the expression of miR-126,miR-326,miRNA-155 and Th cell-associated factor mRNA in BA patients.A rat model of BA was established and rats were given low and high concentrations of TSA intervention and the expression of miR-126,miR-326,miR-155 and Th cell-related genes in each group was detected by PCR technique.The proteins expression of interferon-γ(IFN-γ),GATA binding protein 3(GATA3),retinoic acid receptor-related orphan receptor-γ(ROR-γ)and winged-helix transcription factor 3(Foxp3)were detected by immunohistochemistry.Results The miR-126 and miR-326 and miR-155 mRNA expressions were higher in the BA patient group than those in the healthy people group(1.63±0.30 vs.0.54±0.10,2.25±0.67 vs.1.62±0.39,2.17±0.68 vs.1.75±0.46,P<0.05),and IFN-γand Foxp3 mRNA expressions were lower than those in the healthy people group(0.86±0.19 vs.1.36±0.27,1.01±0.22 vs.1.83±0.30,P<0.05),and ROR-γmRNA expression was higher than that in the normal group(2.79±0.40 vs.1.62±0.38,P<0.05).In the BA rat model,miR-126 mRNA expression in the model group was higher than that in the normal group(2.17±0.39 vs.0.92±0.06,P<0.01),miR-126 mRNA expression in the TSA low-dose group was lower than that in the model group(2.17±0.39 vs.1.24±0.20,P<0.05).GATA3 mRNA expression in the TSA high-dose g

关 键 词：哮喘 微RNA-126 微RNA-326 曲古抑菌素A TH细胞因子 

分 类 号：R562.25[医药卫生—呼吸系统]